Analysis of LECT2 receptor and its signal transduction

LECT2受体及其信号转导分析

• 批准号: 23590275
• 负责人: SATOSHI Yamagoe
• 金额: $ 3.41万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590275/
• 关键词: 生理活性; シグナル伝達; LECT2

Accumulating evidences indicate that LECT2 is associated with several physiological phenomena, but its function is not fully understood. To make clear the function of LECT2, it is important to identify the LECT2 receptor. We performed expression cloning with a retroviral cDNA library constructed from a cell line that highly expressed the LECT2 receptor. Retrovirus infected cells binding LECT2-Fc fusion protein were enriched by cell sorting. The seven cell lines cloned from the enriched cells all had cDNAs derived from the same gene in integrated retrovirus vector. We checked the possibility that this protein was the LECT2 receptor by some experiments. Moreover, we analyzed the phosphoprotein involved in LECT2 signal transduction by western blotting with thirty different kinds of anti-phosphoprotein antibodies, identifying some possible phosphoproteins.

越来越多的证据表明，LECT2与多种生理现象有关，但其功能尚不完全清楚。为了弄清LECT2的功能，鉴定LECT2受体是很重要的。我们用从高表达LECT2受体的细胞系构建的逆转录病毒cDNA文库进行了表达克隆。通过细胞分选富集与LECT2-Fc融合蛋白结合的逆转录病毒感染细胞。从浓缩细胞中克隆的7个细胞系均含有来自整合逆转录病毒载体中相同基因的cDNA。我们通过一些实验验证了该蛋白是LECT2受体的可能性。此外，我们还用30种不同的抗磷蛋白抗体对参与LECT2信号转导的磷酸化蛋白进行了Western blotting分析，鉴定了一些可能的磷化蛋白。

项目成果

Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein : the monomeric forms are stabilized by the zinc ion

白细胞来源的趋化因子 2 是一种锌结合蛋白：单体形式由锌离子稳定

• 发表时间: 2013
• 作者: Okumura A;Suzuki T;Miyatake H;Miyakawa T;Zheng H;Unoki-Kubota H;Dohmae N;Kaburagi Y;Tanokura M;Yamagoe S
• 通讯作者: Yamagoe S

LECT2に関する研究

LECT2研究

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Hepatokine LECT2 causes insulin resistance in skeletal muscle through increased JNK phosphorylation.

肝因子 LECT2 通过增加 JNK 磷酸化导致骨骼肌胰岛素抵抗。

• 发表时间: 2013
• 作者: Fei Lan;Hirohumi Misu;Keita Chikamoto;Hiroaki Takayam1;Akihiro Kikuchi;Satoshi Yamagoe;Shuichi Kaneko;Toshinari Takamura.
• 通讯作者: Toshinari Takamura.

Expression, high-pressure refolding and purification of human leukocyte cell-derived chemotaxin 2 (LECT2).

• DOI: 10.1016/j.pep.2013.01.008
• 发表时间: 2013-04
• 期刊: Protein expression and purification
• 影响因子: 1.6
• 作者: Hai Zheng;T. Miyakawa;Y. Sawano;S. Yamagoe;M. Tanokura