The pharmacological properties of tonic currents mediated by GABAA-Rs in BLA neurons

BLA 神经元中 GABAA-R 介导的强直电流的药理学特性

• 批准号: 23590276
• 负责人: YAMADA JUNKO
• 金额: $ 3.24万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590276/
• 关键词: GABA; 抑制; トニック抑制; 不安; 性差; GABAA受容体; Tonic; Synapse

Synaptically released GABA acting on postsynaptic GABAA receptor produce not only phasic inhibition, but also tonic inhibition by persistent activation of extrasynaptic receptors. This study aimed at examining pharmacological properties of tonic currents mediated by GABAA-Rs in BLA neurons of sex differences using the wild type and PRIP-1 KO mice which is known of downregulation of GABAergic transmission. The application of GABAA-R antagonist bicuculline blocked tonic GABAergic current which was significantly larger than those in KO. Bath application of diazepam and zolpidem causes increase tonic conductance in WT. In contrast, PRIP-1 KO mice showed lower sensitivity to these compounds. These data indicate that tonic GABAergic inhibition exist in principal neurons of the basolateral amygdala in WT mice but PRIP-1 KO mice have imperfection of this inhibitory system.

突触释放的GABA作用于突触后GABAA受体，不仅产生阶段性抑制，而且通过持续激活突触外受体而产生紧张性抑制。本研究旨在使用已知GABA能传递下调的野生型和PRIP-1 KO小鼠来检查性别差异的BLA神经元中由GABAA-Rs介导的紧张电流的药理学性质。应用GABAA-R拮抗剂荷包牡丹碱可阻断紧张性GABA能电流，其电流明显大于KO组。在WT中使用地西泮和唑吡坦会导致强直性电导增加。相比之下，PRIP-1 KO小鼠对这些化合物的敏感性较低。这些数据表明，紧张性GABA能抑制存在于WT小鼠的基底外侧杏仁核的主要神经元，但PRIP-1 KO小鼠有这种抑制系统的不完善。

项目成果

Developmental regulation of KCC2 activity by taurine in a phosphorylation-dependent manner

牛磺酸以磷酸化依赖性方式对 KCC2 活性的发育调节

• 发表时间: 2011
• 作者: Inoue;K.;Furukawa;T.;Yamada;J.;Kumada;T.;Wang;T.;Inoue;R. and Fukuda;A.
• 通讯作者: A.

Dysfunction of tonic GABAergic transmission in PRIP-1 KO mice

PRIP-1 KO 小鼠的强直 GABA 能传递功能障碍

• 发表时间: 2012
• 作者: 1.Zhu G.;Yoshida S.;Migita K.;Yamada J.;Mori F.;Tomiyama M.;Wakabayashi K.;Kanematsu T.;Hirata M.;Kaneko S.;Ueno S and Okada M.;山田順子;山田順子;Junko Yamada;Junko Yamada
• 通讯作者: Junko Yamada

PRIP-1 KOマウスにおける麻酔導入の遅延

PRIP-1 KO 小鼠延迟麻醉诱导

• 发表时间: 2012
• 作者: 二階堂 義和;山田 順子;右田 啓介;柴 祐子;兼松 隆;平田 雅人;上野 伸哉
• 通讯作者: 上野 伸哉

Dysfunction of extrasynaptic GABAergic transmission in PRIP-1 KO mice is associated with an epilepsy phenotype

PRIP-1 KO 小鼠突触外 GABA 能传递功能障碍与癫痫表型相关

• DOI: 10.1124/jpet.111.182386
• 发表时间: 2011
• 期刊: J Pharmacol Exp Ther
• 影响因子: 3.5
• 作者: Zhu G.;Yoshida S.;Migita K.;Yamada J.;Ueno S
• 通讯作者: Ueno S

Cl⁻ homeodynamics in gap junction-coupled astrocytic networks on activation of GABAergic synapses.

• DOI: 10.1113/jphysiol.2013.257162
• 发表时间: 2013-08-15
• 期刊: The Journal of physiology
• 作者: Egawa K;Yamada J;Furukawa T;Yanagawa Y;Fukuda A
• 通讯作者: Fukuda A