Effects of restrained water immersion stress on the metabolism of histamine in the gastric mucosa.

限制水浸应激对胃粘膜组胺代谢的影响。

• 批准号: 23590316
• 负责人: TAKEMURA Motohiko
• 金额: $ 3.49万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590316/
• 关键词: ヒスタミン; 拘束水浸ストレス; 代謝酵素; 生理活性物質; ECL細胞; ヒスチジン脱炭酸酵素

Restrained water immersion stress of 30 min (WRS) in mice and rats caused mild ulcer in the gastric mucosa. Histamine content in the gastric mucosa increased 1.6 and 1.3 times, respectively. Anti-histamine N-methyltransferase (HMT) antibody detected a band in rat kidney, but not in gastric mucosa. Quantitative PCR detected HMT mRNA in gastric mucosa, but the expression level did not change by WRS. Anti-histidine decarboxylase antibody detected a single band in the rat gastric mucosa but the content did not change by WRS. Anti-diamine oxidase antibody failed to detect expression in the gastric mucosa. The phosphorylation level of MAP kinases (ERK1/2, SAPK/KNK, p38) did not change by WRS.

小鼠和大鼠束缚水浸应激30min(WRS)可引起轻度胃黏膜溃疡。胃粘膜组胺含量分别增加1.6倍和1.3倍。抗组胺N-甲基转移酶(HMT)抗体在大鼠肾脏中检测到条带，而在胃粘膜中未检测到条带。定量聚合酶链式反应检测到胃粘膜HMT基因的表达，但WRS对其表达水平无明显影响。抗组氨酸脱羧酶抗体在大鼠胃粘膜中检测到一条单一条带，但WRS未见其含量变化。抗二胺氧化酶抗体未检测到胃粘膜的表达。WRS对MAP激酶(ERK1/2、SAPK/KNK、p38)的磷酸化水平无明显影响。