Different mechanism of carcinogenesis and development in intrahepatic cholangiocarcinoma according to the level of bile ducts

肝内胆管细胞癌是发生于肝脏不同部位的异质性肿瘤。这种异质性取决于ICC的起源是基于大体和组织学检查的胆管树的不同水平。我们检测了氧化应激标志物、Slit-ROBO 1信号通路和葡萄糖代谢在ICC发生和发展中的作用，但未对ICC中胆汁酸及其受体进行研究。结果，1）环氧合酶-2（COX 2）表达与VEGF-C表达和淋巴结转移的存在相关，2）RBE细胞中通过SiRNA降低ROBO 1表达与增殖和迁移以及乳头状淋巴结转移相关，3）葡萄糖转运蛋白-1（GLUT 1）表达与ICC的侵袭性表型相关。

Intrahepatic cholangiocarcinomas (ICCs) consist of heterogenous carcinoma arising from different anatomical sites of the liver. This heterogeneity depends on that the origin of ICCs is different level of biliary tree based on the gross and histologic examination. We examined oxidative stress marker related with carcinogenesis, Slit-ROBO1 signal pathway, and glucose metabolism in carcinogenesis and progression of ICCs, exept we did not perform the investigation of bile acid and its receptor in ICC. As a results, 1) cyclooxygenase-2 (COX2) expression is associated with VEGF-C expression and presence of lymph node metastasis, 2) decreased expression of ROBO1 by SiRNA in RBE cells is associated with proliferation and migration, and pomote lymph node metastasis, and 3) Glucose transporter-1 (GLUT1) expression is related with aggressive phenotype of ICCs.