Different mechanism of carcinogenesis and development in intrahepatic cholangiocarcinoma according to the level of bile ducts

肝内胆管细胞癌(ICCs)是发生于肝脏不同解剖部位的异质性癌。这种异质性取决于大体和组织学检查显示ICCs起源于不同程度的胆道树。我们检测了与ICCs发生发展相关的氧化应激标志物、Sit-Robo1信号通路和糖代谢，但没有对ICC中的胆汁酸及其受体进行研究。因此，1)环氧合酶-2(COX2)的表达与血管内皮细胞生长因子-C(VEGF-C)的表达和有无淋巴结转移有关；(2)rBE细胞中siRNA抑制Robo1的表达与细胞的增殖、迁移和有无淋巴结转移有关；(3)葡萄糖转运体-1(GLUT1)的表达与ICCs的侵袭性表型有关。

Intrahepatic cholangiocarcinomas (ICCs) consist of heterogenous carcinoma arising from different anatomical sites of the liver. This heterogeneity depends on that the origin of ICCs is different level of biliary tree based on the gross and histologic examination. We examined oxidative stress marker related with carcinogenesis, Slit-ROBO1 signal pathway, and glucose metabolism in carcinogenesis and progression of ICCs, exept we did not perform the investigation of bile acid and its receptor in ICC. As a results, 1) cyclooxygenase-2 (COX2) expression is associated with VEGF-C expression and presence of lymph node metastasis, 2) decreased expression of ROBO1 by SiRNA in RBE cells is associated with proliferation and migration, and pomote lymph node metastasis, and 3) Glucose transporter-1 (GLUT1) expression is related with aggressive phenotype of ICCs.