The analysis of the new inhibitory factor for cytomegalovirus in ES/iPS cells

ES/iPS细胞巨细胞病毒新抑制因子分析

• 批准号: 23590445
• 负责人: KAWASAKI Hideya
• 金额: $ 3.33万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590445/
• 关键词: ES; iPS; サイトメガロウイルス; β1 インテグリン; 大脳; レセプター; ES細胞

Pluripotent stem cell (iPS/ES cells) showed resistance to CMV. We established iPS cells from human fibroblast using Yamanka factors. iPS showed remarkable resistance to CMV infection. This is the compatible with previous report (Plos one: 10.1371/journal.pone.0017492). We tried to investigate the new factor for this inhibitory phenomenon.The new factor has not been found yet. Instead, we tried to find known receptor (CD29) distribution for CMV in brain. Endothelial cells, pericytes, meninges, choloid plexus and NSPC all express higher level of CD29 than other area of the brain. Co-expression of higher level of CD29 expressing cells and MCMV infected cells were highly correlated. In the brain. We concluded that cytomegalovirus infection primarily initiates infection from high-level CD29 expressing cells in acute infection phase.

多能干细胞（iPS/ES细胞）对CMV具有抗性。我们使用Yamanka因子从人成纤维细胞建立了iPS细胞。iPS对CMV感染具有显著的抗性。 这与以前的报告（Plos one：10.1371/journal.pone.0017492）一致。我们试图研究这种抑制现象的新因素，但尚未发现新因素。相反，我们试图找到脑中CMV的已知受体（CD 29）分布。内皮细胞、周细胞、脑膜、胆系神经丛和NSPC均表达较高水平的CD 29。较高水平的CD 29表达细胞与MCMV感染细胞的共表达高度相关。在大脑里。我们的结论是，巨细胞病毒感染主要是在急性感染期从高水平的CD 29表达细胞开始感染。

项目成果

Effects of intrapulmonary viral tropism and cytokine expression on the histological patterns of cytomegalovirus pneumonia.

• DOI: 10.1111/j.1440-1827.2012.02849.x
• 发表时间: 2012-09
• 期刊: Pathology international
• 影响因子: 2.2
• 作者: Arai Y;Tsuchida T;Kosugi I;Kawasaki H;Meguro S;Kinoshita M;Baba S;Maeda M;Shinmura Y;Tsutsui Y;Iwashita T
• 通讯作者: Iwashita T

急性期サイトメガロウイルス大脳感染における血行性/髄腔内感染標的細 胞とintegrinβ1との関係

急性期巨细胞病毒脑感染血行/鞘内感染靶细胞与整合素β1的关系

• 发表时间: 2013
• 作者: 河崎 秀陽;鈴木 万幾子;小杉 伊三夫;新井 義文;筒井 祥博;岩下 寿秀
• 通讯作者: 岩下 寿秀

Mouse embryonic stem cells inhibit murine cytomegalovirus infection through a multi-step process.

• DOI: 10.1371/journal.pone.0017492
• 发表时间: 2011-03-02
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kawasaki H;Kosugi I;Arai Y;Iwashita T;Tsutsui Y
• 通讯作者: Tsutsui Y

Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences.

• DOI: 10.1186/1755-1536-6-15
• 发表时间: 2013-08-08
• 期刊: Fibrogenesis & tissue repair
• 作者: Akamatsu T;Arai Y;Kosugi I;Kawasaki H;Meguro S;Sakao M;Shibata K;Suda T;Chida K;Iwashita T
• 通讯作者: Iwashita T

Expression of β‐Adrenoceptor Subtypes in Urothelium, Interstitial Cells and Detrusor of the Human Urinary Bladder

β-肾上腺素受体亚型在人膀胱尿路上皮、间质细胞和逼尿肌中的表达

• DOI: 10.1111/luts.12007
• 发表时间: 2013
• 期刊: LUTS: Lower Urinary Tract Symptoms
• 作者: A. Otsuka;H. Kawasaki;Rikiya Matsumoto;H. Shinbo;Y. Kurita;T. Iwashita;S. Ozono
• 通讯作者: S. Ozono