The analysis of the new inhibitory factor for cytomegalovirus in ES/iPS cells

多能干细胞(iPS/ES细胞)表现出对CMV的抗性。我们用Yamanka因子从人成纤维细胞中建立了iPS细胞。IPS表现出显著的抗CMV侵染能力。这是与前一份报告(PLOS One：10.1371/Journal al.pone.0017492)相兼容的。我们试图研究这种抑制现象的新因素，但还没有发现新的因素。相反，我们试图找到CMV在大脑中的已知受体(CD29)分布。内皮细胞、周细胞、脑膜、脉络丛和NSPC的CD29表达水平均高于脑内其他区域。CD29高表达细胞与MCMV感染细胞共表达高度相关。在大脑里。我们认为，巨细胞病毒感染主要是从急性感染期高表达CD29的细胞开始感染。

Pluripotent stem cell (iPS/ES cells) showed resistance to CMV. We established iPS cells from human fibroblast using Yamanka factors. iPS showed remarkable resistance to CMV infection. This is the compatible with previous report (Plos one: 10.1371/journal.pone.0017492). We tried to investigate the new factor for this inhibitory phenomenon.The new factor has not been found yet. Instead, we tried to find known receptor (CD29) distribution for CMV in brain. Endothelial cells, pericytes, meninges, choloid plexus and NSPC all express higher level of CD29 than other area of the brain. Co-expression of higher level of CD29 expressing cells and MCMV infected cells were highly correlated. In the brain. We concluded that cytomegalovirus infection primarily initiates infection from high-level CD29 expressing cells in acute infection phase.