Development and clinical application of the immunochromatography for a citrin protein

柑橘蛋白免疫层析技术的开发及临床应用

• 批准号: 23590680
• 负责人: IIJIMA Mikio
• 金额: $ 3.08万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590680/
• 关键词: イムノクロマト法; ミトコンドリア; アスパラギン酸・グルタミン酸輸送体

Citrin deficiency causes neonatal intrahepatic cholestasis caused by citrin deficiency, and adult-onset type II citrullinemia. Diagonosis of citrin deficiency is mainly performed by genetic analysis, although the known mutations are not detected in some cases. Western blot analysis is also performed for citrin deficiency. The use of Western blot analysis is very few because it is time-consuming. Immunochromatography is recently developed detection method, which is easy and quick. The aim of this project is to examine citrin protein in oral mucosae by using immunochromatography as an alternative diagnostic method. In this project, the development of immunochromatography for a citrin protein was not completed.

瓜氨酸缺乏症会导致新生儿肝内胆汁淤积症（由瓜氨酸缺乏症引起）和成人型II型瓜氨酸血症。Citrin缺乏症的诊断主要通过遗传分析进行，尽管在某些情况下未检测到已知的突变。还对柠檬酸蛋白缺乏进行蛋白质印迹分析。Western blot分析的使用很少，因为它很耗时。免疫层析法是近年来发展起来的一种简便、快速的检测方法。本研究旨在利用免疫层析技术检测口腔黏膜中的柠檬酸蛋白，作为一种替代的诊断方法。在该项目中，柠檬酸蛋白免疫层析的开发尚未完成。