Inhibitory modulation of the pain sensor protein, TRPA1

疼痛传感器蛋白 TRPA1 的抑制性调节

• 批准号: 23590730
• 负责人: DAI YI
• 金额: $ 3.41万
• 依托单位: Hyogo University of Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590730/
• 关键词: TRPA1; TRPV1; artemin; resveratrol; etodolac; TRPチャネル; DRG; 疼痛; レスベラトロール; Ret; GFRα3

The transient receptor potential (TRP) channel subtype V1 (TRPV1) and A1 (TRPA1) are known to be expressed on sensory neurons and respond to changes in temperature, pH and local application of certain noxious chemicals. In this study, we investigated mechanisms of inhibitory modulation of these TRP channels. Following is the major findings of this study.1. We found that artemin or resveratrol can inhibit TRPA1. The mechanism is likely due to a decreasing of the channel density of TRPA1 on cell membranes. 2. Pinosylvin methyl ether can inhibit TRPV1 by changing the EC50, but not the maximum response of capsaicin-induced currents. 3. Etodolac can direct activate and subsequently desensitize TRPA1 through a covalent modification of cysteine residues. These data indicate that the inhibitory mechanism of modulation of TRP channels are various and complicated.

瞬时受体电位（TRP）通道亚型V1（TRPV1）和A1（TRPA1）已知在感觉神经元上表达，并对温度、pH值和某些有毒化学物质的局部应用的变化作出反应。在这项研究中，我们研究了这些TRP通道的抑制性调制机制。以下是本研究的主要发现.我们发现artemin或resveratrol可以抑制TRPA1。其机制可能是由于TRPA 1在细胞膜上的通道密度降低。2.松素甲醚可通过改变辣椒素诱导电流的EC50而抑制TRPV1，但不能改变辣椒素诱导电流的最大响应。3.依托度酸可通过半胱氨酸残基的共价修饰直接激活并随后使TRPA 1脱敏。这些结果表明，TRP通道的抑制机制是复杂多样的。

项目成果

Etodolac activates and desensitizes transient receptor potential ankyrin 1 through cysteine modification

依托度酸通过半胱氨酸修饰激活瞬时受体电位锚蛋白 1 并使之脱敏

• 发表时间: 2013
• 作者: Wang Shenglan;Noguchi Koichi;Dai Yi
• 通讯作者: Dai Yi

Expression of colony-stimulating factors and their receptors in dorsal root ganglion and spinal cord after peripheral nerve injury

周围神经损伤后背根神经节和脊髓中集落刺激因子及其受体的表达

• 发表时间: 2013
• 作者: OKUBO M;YAMANAKA H;KOBAYASHI K;KANDA H;DAI Y;NOGUCHI K
• 通讯作者: NOGUCHI K

Pinosylvin methyl ether desensitizes the TRPV1 channel

松酚甲醚使 TRPV1 通道脱敏

• 发表时间: 2011
• 作者: Yu L;Noguchi K. Dai Y
• 通讯作者: Noguchi K. Dai Y

ゴシュユの主成分エボジアミンによる一次知覚ニューロンの活性化

吴茱萸的主要成分吴茱萸碱激活初级感觉神经元

• 发表时间: 2011
• 作者: 岩岡恵実子;松吉延之;小暮洋子;青木俊二;野口光一;戴毅
• 通讯作者: 戴毅

Etodolac activates and desensitizes TRPA1

依托度酸激活 TRPA1 并使其脱敏

• 发表时间: 2013
• 作者: 王勝蘭;戴毅;野口光一
• 通讯作者: 野口光一