SEARCHING OF SPECIFIC PROTEINS PRODUCED IN GALLBLADDER CANCER TISSUES FOR THE EARLY DETECTION OF THE CANCER

我们检测和鉴定作为胆囊癌早期检测生物标志物的特异性蛋白质的操作程序如下：从福尔马林固定的石蜡包埋的胆囊癌组织块制备石蜡切片，脱蜡，在显微镜下通过过碘酸-希夫染色对切片进行染色，通过激光显微切割切除组织切片，现场直接消化，使用C18 Stage Tip纯化样品，通过微量二喹啉甲酸蛋白质测定法测量肽溶液的浓度，LC/MS/MS分析，总共收集15个样品：3个来自正常细胞（组I），6个来自癌细胞（组II），6个来自晚期癌细胞（组III）。 每组检测到的蛋白质的平均数量分别为993、1，291和1，109。 在这些蛋白质中，组特异性蛋白质为306、1、148和930。 通过仔细检查这些蛋白质，可能会发现用于胆囊癌早期检测的新生物标志物。

Our operating procedure to detect and identify the specific proteins as the biomarker for the early detection of gallbladder cancer was as follows: preparation of paraffin sections from formalin-fixed paraffin-embedded gallbladder cancer tissue blocks, deparaffinization, stain sections on a microscope by Periodic acid-Schiff stain, excision of tissue section by laser microdissection, on-site direct digestion, purification of the samples using the C18 Stage Tip, concentration measurement of the peptide solutions by micro bicinchoninic acid protein assay, LC/MS/MS analysis.A total of 15 samples were collected: 3 from normal cell (group I), 6 from cancer cell (group II), and 6 from advanced cancer cell (group III). The average numbers of detected proteins from each group were 993, 1,291, and 1,109. In these proteins, the group specific proteins were 306, 1,148, and 930. The new biomarkers for the early detection of gallbladder cancer may be found to examine these proteins closely.