Origin, evolutionaly change and host adaptation of HTLV-1 in East Asia

对从日本各地获得的HTLV-1分离株进行了系统发育分析。因此，从日本获得的分离株大多被归类为JPN或EAS谱系，这意味着日本更有可能从绳文期开始流行。与HTLV-1相反，感染恒河猴的PTLV-1可能具有在血液中释放感染性病毒颗粒的能力。现在我们正试图建立一种检测和定量病毒核糖核酸的方法，以关注病毒物种之间的差异。在20世纪70年代S前后，我们利用从历史样本中检测微量核苷酸的研究环境来检测病理样本中的结核分枝杆菌，并验证了与SM和RFP耐药相关的基因突变，以估计过去耐药的频率。因此，据估计，经病理分析诊断的结核病患者中，超过一半的人患有耐药株。

Phylogenetic analyses of HTLV-1 isolates obtained from various parts of Japan were conducted. As a result, most of the isolates obtained from Japan were classified into the JPN or the EAS lineages, which means that there were more likely to be endemic in Japan from the Jomon period.In contrast to HTLV-1, PTLV-1 infecting Macaca fuscata may have an ability to release infective viral particles in blood. Now we are trying to establish a method to detect and quantify viral RNA to focus on difference between the viral species.A research environment to detect a trace level of nucleotides from historical samples was used to detect DNA of Mycobacterium tuberculosis from pathological samples around 1970's. Genetic mutations related to SM and RFP resistances were verified to estimate the frequency of drug resistance in the past. As a result, it is estimated that more than half of tuberculosis patients diagnosed by pathological analysis were suffered from SM resistant strains.