Role of iron as an detrimental influence on inflammatory bowel disease -analysis based on enteric microbiota and iron-related molecules

铁对炎症性肠病的有害影响——基于肠道微生物群和铁相关分子的分析

• 批准号: 23590940
• 负责人: MATSUURA MINORU
• 金额: $ 3.16万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590940/
• 关键词: 炎症性腸疾患; 環境因子; 鉄; 腸内細菌

Diet is a potential environmental influence in inflammatory bowel diseases (IBD). Enteric bacteria are required to induce and perpetuate intestinal inflammation in IBD, and iron is essential for the growth and virulence of bacteria. IL-10 deficient mice were fed normal- or low-iron diets. Histologic inflammation in the low-iron diet group was ameliorated and colonic IL-12p40 and MLN IL-17 secretion was lower in mice fed low-iron diet. There was no significant difference in concentrations of cultivable cecal bacteria, while T-RFLP analysis revealed distinct difference in the profile between two groups. The intracellular survival of iron-loaded E. coli in J774 macrophage cells was significantly enhanced compared to non-iron-loaded E. coli. TNF secretion from J774 cells phagocytosing iron-loaded E. coli was higher than J774 cells phagocytosing non-iron-loaded E. coli. Dietary iron potentiates intestinal inflammation by alteration of growth and virulence of enteric microbiota.

饮食是炎症性肠病（IBD）的潜在环境影响。肠道细菌是诱导和维持IBD肠道炎症所必需的，而铁对细菌的生长和毒力至关重要。IL-10缺陷小鼠喂食正常或低铁饮食。低铁饮食组的组织学炎症得到改善，并且饲喂低铁饮食的小鼠结肠IL-12 p40和MLN IL-17分泌较低。盲肠细菌培养浓度无显著性差异，但T-RFLP分析显示两组间差异显著。铁负载的E. coli在J774巨噬细胞中的表达显著高于未负载铁的E.杆菌J774细胞吞噬负载铁的E. coli对非铁负载E. coli的吞噬能力高于J774细胞。杆菌膳食铁通过改变肠道微生物群的生长和毒力来增强肠道炎症。

项目成果

Effect of intensive granulocyte and monocyte adsorptive apheresis in patients with ulcerative colitis positive for cytomegalovirus

• DOI: 10.1016/j.crohns.2012.12.003
• 发表时间: 2013-11-01
• 期刊: JOURNAL OF CROHNS & COLITIS
• 影响因子: 8
• 作者: Fukuchi, Takumi;Nakase, Hiroshi;Ashida, Kiyoshi
• 通讯作者: Ashida, Kiyoshi

Long-term follow-up in patients with ulcerative colitis treated with tacrolimus : early and long-term data from a retrospective observational study

他克莫司治疗溃疡性结肠炎患者的长期随访：回顾性观察研究的早期和长期数据

• 发表时间: 2013
• 作者: Matsuura M;Nakase H;Yoshino T;Chiba T
• 通讯作者: Chiba T

Prior use of immunomodulatory drugs improves the clinical outcome of endoscopic balloon dilation for intestinal stricture in patients with Crohn's disease.

预先使用免疫调节药物可改善克罗恩病患者肠道狭窄的内镜球囊扩张的临床结果。

• DOI: 10.1111/den.12029
• 发表时间: 2013
• 期刊: Digestive Endoscopy
• 影响因子: 5.3
• 作者: Shinzaki S;他13名;Naka T;Plevy S E;Takehara T;Miyoshi E.;Miyamoto S.;Honzawa Y
• 通讯作者: Honzawa Y

Microbial balances altered by restriction of dietary iron ameliorated immune-mediated colitis

限制膳食铁改变微生物平衡可改善免疫介导的结肠炎

• 发表时间: 2014
• 作者: Toyonaga T;Nakase H;Matsuura M;Yamada S;Minami S;Honzawa Y;Yoshino T;Okazaki K;Chiba T
• 通讯作者: Chiba T

Fecal lipocalin2 reflects colonic inflammation in murine colitis model : A possible role of measuring fecal lipocalin2 as a biomarker of IBD

粪便脂质运载蛋白2反映了小鼠结肠炎模型中的结肠炎症：测量粪便脂质运载蛋白2作为IBD生物标志物的可能作用

• 发表时间: 2013
• 作者: Toyonaga T;Matsuura M;Yamada S;Minami S;Honzawa Y;Yoshino T;Nakase H;Okazaki K;Chiba T
• 通讯作者: Chiba T