The role of cell cycle regulator Cdh1 in carcinogenesis and development of gastrointestinal cancer.

siRNA tequnique抑制结肠癌细胞系Cdh 1的表达这些细胞显示出细胞骨架异常，肌动蛋白丝减少。在伤口愈合试验中，Cdh 1抑制细胞显示出比对照细胞更高的运动性。接下来，我们用基因陷阱tequnique抑制Cdh 1的一个磷酸化位点产生异源小鼠。这些小鼠后代的基因分型如下：野生型：异源型：同源型=13：32：0。这些结果表明，Homo抑制Cdh 1磷酸化位点是小鼠胚胎的致死事件，我们正在研究其致死时间和死亡原因。

Suppress the expression of Cdh1 with siRNA tequnique in colon cancer cell line. These cells revealed cytoskeletal abnormality with decreased actin filament.In the analysis of cell motility with the wound healing assay, Cdh1 suppresse cells showed high motility compared with the control cells.Next, we generated heterogenous mice which one of the phosphorylation site of Cdh1 was suppressed with gene trap tequnique.Genotyping of offspring of these mice was following as wild type: hetero: homo=13:32:0. These results indicated that homo suppression of Cdh1 phosphorylation site was embryonic lethal event in mice.Now,we are studying about the timing of lethality and cause of death.