The role of cell cycle regulator Cdh1 in carcinogenesis and development of gastrointestinal cancer.

利用siRNAteqUnique抑制结肠癌细胞中CDH1的表达。在创伤修复实验的细胞运动性分析中，CDH1抑制细胞表现出比对照细胞高的运动性。接下来，我们产生了CDH1磷酸化位点之一被基因TRAP teq唯一抑制的异种小鼠。这些小鼠的后代的基因分型如下：野生型：异型：Homo=13：32：0。这些结果表明，CDH1磷酸化位点的同源抑制是小鼠胚胎死亡事件。现在，我们正在研究死亡的时间和死亡原因。

Suppress the expression of Cdh1 with siRNA tequnique in colon cancer cell line. These cells revealed cytoskeletal abnormality with decreased actin filament.In the analysis of cell motility with the wound healing assay, Cdh1 suppresse cells showed high motility compared with the control cells.Next, we generated heterogenous mice which one of the phosphorylation site of Cdh1 was suppressed with gene trap tequnique.Genotyping of offspring of these mice was following as wild type: hetero: homo=13:32:0. These results indicated that homo suppression of Cdh1 phosphorylation site was embryonic lethal event in mice.Now,we are studying about the timing of lethality and cause of death.