Novel biomarker for evaluation of autophagic dysfunction in the liver

我们发现自噬功能障碍改变了核膜蛋白的表达。自噬功能障碍时细胞核膜蛋白的增加可能是评价自噬功能障碍的一个有用的生物标志物。另一方面，在约65%的NAFLD中检测到肝细胞中的p62聚集，这与组织蛋白酶B、D和L表达的抑制相关。在NAFLD患者中，p62聚集与血清丙氨酸氨基转移酶值和NAS的炎症活性相关。这些结果表明，抑制自噬蛋白水解的肝脂肪变性参与了NAFLD的发病机制。

We found that autophagic dysfunction alters the expression of nuclear membrane protein. Nuclear membrane proteins increased in autophagic dysfunction might be an useful biomarker to evaluate autophagic dysfunction. On the other hand, aggregation of p62 in hepatocytes was detected in about 65% of NAFLD correlatively to suppression of cathepsin B, D and L expression. In NAFLD patients, p62 aggregation was correlated with serum alanine aminotransferase value and inflammatory activity by NAS. These findings indicate that the suppression of autophagic proteolysis by hepatic steatosis is involved in the pathogenesis of NAFLD.