The role of cancer associated fibroblast in non small cell lung cancer

癌症相关成纤维细胞在非小细胞肺癌中的作用

• 批准号: 23591161
• 负责人: TOGO Shinsaku
• 金额: $ 3.49万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591161/
• 关键词: 癌関連線維芽細胞; 非小細胞肺癌; EMT; 癌微小環境

The activated fibroblasts that are found in association with cancer cells are known as cancer-associated fibroblasts (CAFs). CAFs probably promote tumor progression through specific communications with cancer cells. Methods: Control: derived from normal lung tissue and CAFs: derived from tumor in same donors were prepared from surgical tissues. The ability of fibroblasts to mediate contraction of three dimensional type I collagen gels and Fibroblasts chemotaxis were evaluated. Fibroblasts cell viability by chemoreagents was assayed. Primary fibroblasts preserved their ability to induce migration and collagen gel contraction, which was greater with CAFs than NFs and the condition media from CAFs stimulated more A549 chemotaxis. The cell viability of CAFs against CDDP showed higher than that of controls. These results showed that CAFs can maintain the phenotypic properties of myofibroblasts and be further activated the myofibroblast activity interaction with carcinoma cells.

与癌细胞相关的活化成纤维细胞被称为癌症相关成纤维细胞（CAF）。CAF可能通过与癌细胞的特异性通讯促进肿瘤进展。方法：对照：来自正常肺组织，CAFs：来自同一供体的肿瘤，来自手术组织。评价成纤维细胞介导三维I型胶原凝胶收缩的能力和成纤维细胞趋化性。通过化学试剂测定成纤维细胞的细胞活力。原代成纤维细胞保持其诱导迁移和胶原凝胶收缩的能力，CAFs的这种能力大于NF，CAFs的条件培养基刺激更多的A549趋化性。CAFs对CDDP的细胞活力高于对照组。 这些结果表明，CAFs能够保持肌成纤维细胞的表型特性，并进一步激活肌成纤维细胞活性与癌细胞的相互作用。

项目成果

肺癌と癌関連線維芽細胞

肺癌和癌症相关成纤维细胞

• 发表时间: 2012
• 作者: 十合晋作;高橋和久
• 通讯作者: 高橋和久

Carcinoma-associated fibroblasts are a promising therapeutic target.

• DOI: 10.3390/cancers5010149
• 发表时间: 2013-01-31
• 期刊: Cancers
• 影响因子: 5.2
• 作者: Togo S;Polanska UM;Horimoto Y;Orimo A
• 通讯作者: Orimo A

非小細胞肺癌間質内における筋線維芽細胞の機能解析

非小细胞肺癌基质内肌成纤维细胞的功能分析

• 发表时间: 2011
• 作者: 長浜久美;十合晋作
• 通讯作者: 十合晋作

非小細胞肺癌間質内における筋線維芽細胞の機能解析の検討

非小细胞肺癌基质内肌成纤维细胞的功能分析检查

• 发表时间: 2011
• 作者: Ohhashi K;Sato A;Takada T;Ishii H;Nakata K;Tazawa R.;十合晋作
• 通讯作者: 十合晋作

Carcinoma-Associated Fibroblasts are a promising therapeuic target. Review

癌相关成纤维细胞是一个有前途的治疗靶点。

• 发表时间: 2013
• 期刊: Cancers
• 影响因子: 5.2
• 作者: S.Togo;Orimo. A et al
• 通讯作者: Orimo. A et al