The role of cancer associated fibroblast in non small cell lung cancer

与癌细胞相关的活化成纤维细胞被称为癌症相关成纤维细胞（CAFs）。CAFs可能通过与癌细胞的特定通信促进肿瘤进展。方法：对照：取自正常肺组织；CAFs：取自同一供体的肿瘤，取自手术组织。对成纤维细胞介导三维I型胶原凝胶收缩的能力和成纤维细胞趋化性进行了评价。用化学试剂检测成纤维细胞活力。原代成纤维细胞保留了诱导迁移和胶原凝胶收缩的能力，CAFs比NFs更强，CAFs的条件培养基刺激了更多的A549趋化性。CAFs对CDDP的细胞活力高于对照。这些结果表明，CAFs可以维持肌成纤维细胞的表型特性，并进一步激活肌成纤维细胞与癌细胞相互作用的活性。

The activated fibroblasts that are found in association with cancer cells are known as cancer-associated fibroblasts (CAFs). CAFs probably promote tumor progression through specific communications with cancer cells. Methods: Control: derived from normal lung tissue and CAFs: derived from tumor in same donors were prepared from surgical tissues. The ability of fibroblasts to mediate contraction of three dimensional type I collagen gels and Fibroblasts chemotaxis were evaluated. Fibroblasts cell viability by chemoreagents was assayed. Primary fibroblasts preserved their ability to induce migration and collagen gel contraction, which was greater with CAFs than NFs and the condition media from CAFs stimulated more A549 chemotaxis. The cell viability of CAFs against CDDP showed higher than that of controls. These results showed that CAFs can maintain the phenotypic properties of myofibroblasts and be further activated the myofibroblast activity interaction with carcinoma cells.