Identification of microRNAs involved in the development of diabetic nephropathy

鉴定参与糖尿病肾病发展的 microRNA

• 批准号: 23591177
• 负责人: FUJITA HIROKI
• 金额: $ 3.41万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591177/
• 关键词: 糖尿病性腎症; マイクロRNA

MicroRNAs (miRs) are a family of non-coding RNAs and inhibit target gene expression by causing messenger RNA degradation or by blocking protein translation. We analysed the specific expression of miRs by microarray and real-time RT-PCR using RNA extracted from whole kidneys and isolated glomeruli in two Akita diabetic mouse models which have different susceptibility to diabetic nephropathy, nephropathy-resistant C57BL/6-Akita and nephropathy-prone KK/Ta-Akita. Several miRs whose expression levels are different between C57BL/6-Akita and KK/Ta-Akita were identified in both whole kidney and glomerulus. These results indicate that the identified miRs may play an important role in the development and progression of diabetic nephropathy.

microRNA（miRs）是一类非编码RNA家族，通过降解信使RNA或阻断蛋白质翻译来抑制靶基因的表达。我们使用从两种秋田糖尿病小鼠模型（肾病抵抗的C57 BL/6-秋田和肾病倾向的KK/Ta-秋田）的整个肾脏和分离的肾小球提取的RNA，通过微阵列和实时RT-PCR分析miR的特异性表达。在整个肾脏和肾小球中鉴定了C57 BL/6-秋田和KK/Ta-秋田之间表达水平不同的几种miR。这些结果表明，所鉴定的miRs可能在糖尿病肾病的发生和进展中起重要作用。

项目成果

マイクロアレイ解析による糖尿病性腎症感受性マイクロRNAの同定

通过微阵列分析鉴定易患糖尿病肾病的 microRNA

• 发表时间: 2011
• 作者: 藤田浩樹;森井宰;藤田麻貴;高嶋悟;小川和孝;三ケ田敦史;菅沼由美;清水尚子;清水辰徳;佐藤雄大;細葉美穂子;月山克史;成田琢磨;山田祐一郎
• 通讯作者: 山田祐一郎