Regulation of Renal Stem Cell Number by Endothelial Cell-derived Factors

内皮细胞衍生因子对肾干细胞数量的调节

• 批准号: 23591181
• 负责人: MAESHIMA AKITO
• 金额: $ 3.16万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591181/
• 关键词: 腎幹細胞; 尿細管再生; 血管内皮由来因子; 血管内皮細胞; 加齢

We examined the role of endothelial cell-derived factors in growth and differentiation of renal stem cell. Using BrdU labeling method, renal stem cells could be detected as BrdU-positive cells. The number of renal stem cells as well as the interstitial peritubular capillary area decreased with aging. When isolated renal stem cells were co-cultured with HUVEC, the number of renal stem cells significantly increased. HGF induced tube formation in human RPTEC. When RPTEC were co-cultured with HUVEC, tube formation was significantly enhanced. Multiple receptor tyrosine kinases were phosphorylated in RPTEC during tube formation. Collectively, these data suggest the presence of stem cell activators in the endothelial cell-derived factors.

我们研究了内皮细胞衍生因子在肾干细胞生长和分化中的作用。 用BrdU标记法可检测到肾干细胞为BrdU阳性细胞。 随着年龄的增长，肾间质干细胞数量和肾小管周毛细血管面积减少。 当分离的肾干细胞与HUVEC共培养时，肾干细胞的数量显著增加。 HGF诱导人RPTEC中的管形成。 当RPTEC与HUVEC共培养时，管形成显著增强。 多个受体酪氨酸激酶磷酸化在RPTEC管形成过程中。 总的来说，这些数据表明在内皮细胞衍生因子中存在干细胞活化剂。

项目成果

Age-related decline in LRTCｓ

LRTC 与年龄相关的下降

• 发表时间: 2012
• 期刊: Am J Physiol Renal Physiol
• 作者: Miya M;Maeshima A;Mishima K;Sakurai N;Ikeuchi H;Kuroiwa T;Hiromura K;Nojima Y.;Masaaki Miya
• 通讯作者: Masaaki Miya

Quantitative evaluation of epithelial-mesenchymal transition during renal fibrosis

肾纤维化过程中上皮间质转化的定量评估

• 发表时间: 2013
• 作者: Nakasatomi M;Maeshima A;Mishima K;Sakurai N;Ikeuchi H;Sakairi T;Kaneko Y;Hiromura K;Nojima Y
• 通讯作者: Nojima Y

腎臓の線維化と再生を担う細胞群を探る「腎尿細管の修復・再生メカニズム」

“肾小管的修复和再生机制”探索负责肾纤维化和再生的细胞群

• 发表时间: 2011
• 作者: Kayakabe K;Kuroiwa T;Sakurai N;Ikeuchi H;Anastasie KT;Sakairi T;Maeshima A;Hiromura K;Nojima Y;前嶋明人
• 通讯作者: 前嶋明人

Involvement of N-type Ca2+ channels in the fibrotic process of the kidney in rats

• DOI: 10.1152/ajprenal.00561.2012
• 发表时间: 2013-03-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
• 影响因子: 4.2
• 作者: Mishima, Keiichiro;Maeshima, Akito;Nojima, Yoshihisa
• 通讯作者: Nojima, Yoshihisa

腎臓再生に関する最近の知見

关于肾脏再生的最新发现

• 发表时间: 2012
• 作者: Akito Maeshima;Masaaki Miya;Keiichiro Mishima;Noriyuki Sakurai;Hidekazu Ikeuchi;Toru Sakairi;Yoriaki Kaneko;Keiju Hiromura;Yoshihisa Nojima;前嶋明人
• 通讯作者: 前嶋明人