Adipose-derived mesenchymal stem cells transplantation facilitate experimental peritoneal fibrosis repair by suppressing epithelial–mesenchymal transition

脂肪间充质干细胞移植通过抑制上皮间质转化促进实验性腹膜纤维化修复

• 批准号: 23591226
• 负责人: HAMADA Chieko
• 金额: $ 3.08万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591226/
• 关键词: peritoneal fibrosis; mesenchymal stem cell; EMT; angiogenesis; 腹膜線維化; 脂肪幹細胞; 上皮間葉系移行; 血管新生; 肪幹細胞; 被嚢性腹膜硬化症; VEGF; TNF-α; IL-1β; 再生医療; HGF; basic FGF

Peritoneal fibrosis (PF) remains a serious complication of PD that involves in the morphological and functional deteriorations of peritoneum. It is still unclear the effect of adipose-derived mesenchymal stem cell (ASC) to the PF. The effect of ASC transplantation was compared with peritoneal mesothelial cell(PMC) during repair in chlorhexidine gluconate (CG)-induced PF rats. To induce the PF rat, continuous-infusion pumps containing 8 % CG were placed into the abdominal cavity for 21 days. PMCs and ASCs were injected at day 22 or 29, and sacrificed at day 35. ASC transplantation significantly facilitated peritoneal repair in the dose dependent manner regardless of transplant timing. The mRNA expression of fibrosis-related factors were significantly suppressed. ASC transplantation induced the over-expression of angiogenesis-related factors.It appears that the ASC might be an effective strategy for morphological retrieval in the PF through the anti-inflammatory and anti-EMT effect.

腹膜纤维化(PF)仍然是PD的严重并发症，涉及腹膜的形态和功能的恶化。脂肪间充质干细胞(ASC)对PF的作用尚不清楚。比较葡萄糖酸洗必泰(CG)诱导的PF大鼠修复过程中ASC移植与腹膜间皮细胞(PMC)修复的效果。将含8%CG的持续输液泵放置于大鼠腹膜腔内21天，建立肺纤维化大鼠模型。分别于第22、29天注射PMC和ASCs，第35天处死。ASC移植以剂量依赖的方式显着促进腹膜修复，与移植时机无关。肝纤维化相关因子的mRNA表达受到明显抑制。ASC移植诱导了血管生成相关因子的过度表达，ASC可能是通过抗炎和抗EMT作用恢复PF形态的一种有效策略。

项目成果

クロルヘキシジン誘発腹膜線維症モデルラットにおける脂肪幹細胞移植

脂肪干细胞移植在洗必泰诱导的腹膜纤维化模型大鼠中的应用

• 发表时间: 2012
• 作者: Shigematsu T;Negi S; COLC Research Group;Tojo A;若林啓一
• 通讯作者: 若林啓一

Facilitative effects of transplanted adipose-derived mesenchymal stem cells during repair in chlorhexidine -induced peritoneal fibrosis rats

移植的脂肪间充质干细胞对洗必泰诱导的腹膜纤维化大鼠修复过程中的促进作用

• 发表时间: 2012
• 作者: K. Hayashi;H. Sasamura;T. Azegami;H. Itoh;KEIICHI WAKABAYASHI
• 通讯作者: KEIICHI WAKABAYASHI

Facilitative effects of transplanted adipose-derived mesenchymal stem cells during repair in chlorhexidine-induced peritoneal fibrosis rats

移植的脂肪间充质干细胞对洗必泰诱导的腹膜纤维化大鼠修复过程中的促进作用

• 发表时间: 2012
• 作者: 若林啓一;濱田千江子;神田怜生;中野貴則;井尾浩章;堀越哲;富野康日己
• 通讯作者: 富野康日己

Facilitative effects of transplanted adipose-derived mesenchymal stem cells during repair in chlorhexidine(CH)-induced peritoneal fibrosis rats

移植的脂肪间充质干细胞对洗必泰（CH）诱导的腹膜纤维化大鼠修复过程中的促进作用

• 发表时间: 2013
• 作者: 若林啓一;濱田千江子;神田怜生;中野貴則;井尾浩章;堀越哲;富野康日己
• 通讯作者: 富野康日己