The effects of a SNP in 3'UTR on the microRNA-mediated regulation of aipha-Synuclein gene

3UTR 中的 SNP 对 microRNA 介导的 aipha-Synuclein 基因调控的影响

• 批准号: 23591257
• 负责人: MIZUNO Hideya
• 金额: $ 3.33万
• 依托单位: Mukogawa Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591257/
• 关键词: 神経変性疾患; パーキンソン病; SNP; マイクロRNA; αシヌクレイン

a SNP in 3'UTR of alpha-Synuclein (aSyn) gene (rs356165) contributes to the increase of the risk for sporadic Parkinson's disease (PD). We searched the miRNAs regulating aSyn expression the regulation of which is affected by the SNP. We made vectors inserting aSyn 3'UTR including G type (major allele, high risk type for PD) or A type (minor allele) of the SNP. The vectors was introduced into cultured cells with miRNAs and the reporter activities and aSyn protein expression were measured by luciferase assay or western blotting. miR-7 and -153 inhibited the aSyn expression. Howerver, there was no difference in the negative regulation by miR-7 or -153 between G and A type

α-突触核蛋白（aSyn）基因（rs356165）3 'UTR的SNP有助于增加散发性帕金森病（PD）的风险。 我们搜索了调节aSyn表达的miRNA，其调节受SNP影响。我们制备了插入aSyn 3 'UTR的载体，包括G型（主要等位基因，PD的高风险型）或A型（次要等位基因）的SNP。将载体导入具有miRNA的培养细胞中，并通过荧光素酶测定或蛋白质印迹法测量报告子活性和aSyn蛋白表达。miR-7和-153抑制aSyn表达。而miR-7和-153对G型和A型细胞的负调控作用无差异

项目成果

microRNAと低分子量Gタンパク質Rhesが細胞表現型に与える影響について

microRNA和低分子量G蛋白Rhes对细胞表型的影响

• 发表时间: 2014
• 作者: 水野英哉;横山千恵子;鮫島由香;河端真実;中林利克
• 通讯作者: 中林利克