Tissue engineered blood vessel sheet to prevent cerebral infarction

组织工程血管片预防脑梗塞

• 批准号: 23591281
• 负责人: ITOH Yoshiaki
• 金额: $ 3.66万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591281/
• 关键词: 人工血管; 脳虚血治療; 血管新生; 内皮細胞; 治療的血管新生; 人工血管シート; 脳血管内皮細胞; 主幹動脈閉塞症; 再生医療; 移植医療; 血管再生; 血管内皮細胞; 脳虚血; 周皮細胞; 治療

Background: Therapeutic angiogenesis is now actively studied as a method to aleviate cerebral infarction after major artery occlusion. We developed a tissue engineered blood vessel sheet to facilitate bypass flow in an animal model of arterial occlusion.Methods: Human brain micirovessel endothelial cells were labeled with GFP and were seeded in collagen membrane together with 10T1/2 cells, mesenchymal precurser cells. After craniectomy in immunosuppressed mouse (SCID mouse), the membrane with endothelial cells were placed over the cortex and pia matter.Results: Immature vessel structure was observed after 3 days and blood flow from cortical arteriole was observed after 5 days. Size of cerebral infarct induced by permanent MCA occlusion became much smaller than control.Conclusion: Tissue engineered pial vessel may be useful in prevention of cerebral infarction after major artery occlusion.

背景：治疗性血管生成作为一种减轻大动脉闭塞后脑梗死的方法正在被积极研究。方法：将绿色荧光蛋白（GFP）标记的人脑微血管内皮细胞与10 T1/2细胞、间充质干细胞一起接种于胶原膜上，用免疫组织化学方法检测血管内皮细胞的表达。结果：免疫抑制小鼠颅骨切除术后3d可见未成熟血管结构，5d可见皮质小动脉血流。结论：组织工程软脑膜血管可有效预防大脑中动脉永久性闭塞后的脑梗死。

项目成果

マウス一側総頸動脈閉塞後に観察された血管新生を伴わない毛細血管の動脈化と側副血行路の発達

小鼠单侧颈总动脉闭塞后观察到无血管生成和侧枝血液循环发展的毛细血管动脉化

• 作者: 伊藤義彰;郭淮莲;鳥海春樹;山田哲;小泉健三;冨田裕
• 通讯作者: 冨田裕

虚血組織に対する治療的血管新生

缺血组织的治疗性血管生成

• 发表时间: 2012
• 期刊: 脳循環代謝
• 作者: 伊藤義彰;黒井俊哉;鳥海春樹;海老根妙子;畝川美悠紀;山田哲;此枝史恵;鈴木則宏
• 通讯作者: 鈴木則宏

Astrocytes and pericytes cooperatively maintain a capillary-like structure composed of endothelial cells on gel matrix

• DOI: 10.1016/j.brainres.2011.06.039
• 发表时间: 2011-08-11
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Itoh, Yoshiaki;Toriumi, Haruki;Suzuki, Norihiro
• 通讯作者: Suzuki, Norihiro

Propagation of changes in diameter of pial a rteries and cerebral blood flow following cortical spreading depression in anesthetized mice.

麻醉小鼠皮质扩散抑制后软脑膜动脉直径和脑血流量变化的传播。

• 发表时间: 2013
• 作者: Unekawa M;Tomita Y;Toriumi H;Osada T;Masamoto K;Itoh Y;Kanno I;Suzuki N.
• 通讯作者: Suzuki N.

脳梗塞急性期の抗凝固療法・抗血小板療法

脑梗塞急性期的抗凝治疗/抗血小板治疗

• 发表时间: 2013
• 期刊: 臨床神経
• 作者: Murata K;Kouda K;Tajima F;Kondo T;伊藤義彰
• 通讯作者: 伊藤義彰