Tissue engineered blood vessel sheet to prevent cerebral infarction

点击翻译按钮获取中文摘要

Background: Therapeutic angiogenesis is now actively studied as a method to aleviate cerebral infarction after major artery occlusion. We developed a tissue engineered blood vessel sheet to facilitate bypass flow in an animal model of arterial occlusion.Methods: Human brain micirovessel endothelial cells were labeled with GFP and were seeded in collagen membrane together with 10T1/2 cells, mesenchymal precurser cells. After craniectomy in immunosuppressed mouse (SCID mouse), the membrane with endothelial cells were placed over the cortex and pia matter.Results: Immature vessel structure was observed after 3 days and blood flow from cortical arteriole was observed after 5 days. Size of cerebral infarct induced by permanent MCA occlusion became much smaller than control.Conclusion: Tissue engineered pial vessel may be useful in prevention of cerebral infarction after major artery occlusion.