Toward novel molecular targeting strategies for the metabolic syndrome

代谢综合征的新型分子靶向策略

• 批准号: 23591318
• 负责人: KISHIDA Tsunao
• 金额: $ 3.33万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591318/
• 关键词: 糖脂質代謝; ﾒﾀﾎﾞﾘｯｸｼﾝﾄﾞﾛｰム; iPS細胞; 褐色脂肪細胞; メタボリックシンドローム; ダイレクトコンヴァージョン; ﾒﾀﾎﾞﾘｯｸｼﾝﾄﾞﾛｰﾑ

In rodents and other animals, brown adipocytes contribute to energy expenditure and heat production, but brown adipocytes of humans have not been characterized in detail, mainly because functional human brown adipocytes that can be propagated in culture have not been available for experimental use so far. We recently established novel procedures to induce human and murine brown adipocytes. In this study we examined gene expression profiles, epigenetic states, and metabolic activities of the brown adipocytes, while we also analyzed regulation of their activities and molecular mechanisms of their induction. The present findings have important implication to novel molecular targeting therapy against metabolic syndromes.

在啮齿动物和其他动物中，棕色脂肪细胞有助于能量消耗和产热，但人类的棕色脂肪细胞尚未被详细描述，主要是因为可以在培养中繁殖的功能性人类棕色脂肪细胞迄今尚未可用于实验用途。我们最近建立了诱导人类和小鼠棕色脂肪细胞的新方法。在这项研究中，我们检测了棕色脂肪细胞的基因表达谱、表观遗传状态和代谢活性，同时也分析了它们的活性调节和诱导的分子机制。本研究结果对代谢综合征的分子靶向治疗具有重要意义。

项目成果

Sonoporation-mediated transduction of siRNA targeting TNF alpha ameliorated experimental arthritis

声孔介导的靶向 TNF α 的 siRNA 转导可改善实验性关节炎

• 发表时间: 2014
• 作者: Inoue H;Arai Y;Nakagawa S;Saito M ,Tsuchida S;Inoue A;Matsuki T;Morihara T;Shirai T;Mazda;O;Kubo T
• 通讯作者: Kubo T

褐色脂肪細胞及びその調製方法

棕色脂肪细胞及其制备方法

• 发表时间: 2012

Anti-inflammatory effects of lansoprazole by suppressing ROS production in murine macrophage RAW 264.7 cells

兰索拉唑通过抑制小鼠巨噬细胞 RAW 264.7 细胞中 ROS 的产生发挥抗炎作用

• 发表时间: 2014
• 作者: S. Ichimaru;S. Nakagawa;Y. Arai;S. Tsuchida;H. Inoue;T. Matsuki;Y. Mikami;T. Ikeda;R. Oda;O. Mazda;T. Kubo
• 通讯作者: T. Kubo

Effect of mechanical stress on the hypertrophic differentiation related gene expression in cultured chondrocytes

机械应力对培养软骨细胞肥大分化相关基因表达的影响

• 发表时间: 2013
• 作者: Inoue H;Arai Y;Terauchi R;Nakagawa S;Saito M;Hiraoka N;Tsuchida S;Matsuki T;Mazda Osam;Kubo T
• 通讯作者: Kubo T

Femoral perfusion after electromagnetic fields stimulation to the steroid-induced osteonecrosis model.

电磁场刺激类固醇诱导的骨坏死模型后的股骨灌注。

• 发表时间: 2014
• 作者: Sasaki K;Inose H;Kawabata S;Yoshii T;Kato T;Saito M;Okawa A.;Ikegami A. et al
• 通讯作者: Ikegami A. et al