Analysis of the function of LR11 on normal leukocytes and leukemia cells

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We have identified and characterized a regulator of uPAR function, LR11 (also called SorLA or SORL1), in vascular smooth muscle cells (SMCs). Both LR11 mRNA and cell surface protein levels are elevated in immature leukemic cells, in turn leading to increased levels of sLR11 in acute leukemias. Thus, we analyzed the function of LR11 on normal hematopoietic cells and leukemia cells.Results: K562 cells express very low levels of LR11 protein, but there was no difference in cell growth between mock transfected cells and LR11-overexpressing (LR11-ox) K562 cells in vitro. The percent of leukemia cells in PB of the mice transplanted with LR11-ox K562 cells on 4 weeks after transplantation were significantly higher than those of mice transplanted with mock cells. All the recipient mice transplanted with control U937 cells were died of leukemia by 3 weeks after transplantation, whereas the survival of mice transplanted with LR11-KD U937 cells were prolonged significantly.