Identification of cellular genes associated with pathogenesis of chronic active Epstein-Barr virus infection

慢性活动性 Epstein-Barr 病毒感染 (CAEBV) 的发病机制仍不清楚。先前的研究已经确定了许多与 CAEBV 相关的候选细胞基因。然而，关于 CAEBV 患者新鲜分离细胞的基因表达水平的数据很少。在本研究中，外周血样本取自 CAEBV 患者。我们使用专注于 T 细胞和 B 细胞激活的 PCR 阵列分析研究了 CAEBV 中的宿主细胞基因表达谱。基于PCR阵列分析和随后的定量实时PCR测定，我们发现CAEBV患者中有四个基因（IL-10、IL-2、IFNGR1和INHBA）上调，这四个基因与对照受试者相比表现出十倍的差异。这些基因可能与炎症反应和细胞增殖有关，并且可能有助于 CAEBV 的发生和进展。

The pathogenesis of chronic active Epstein-Barr virus infection (CAEBV) remains unclear. Previous studies have determined a number of candidate cellular genes associated with CAEBV. However, few data are available on the gene expression levels in freshly isolated cells from patients with CAEBV. In the present study, peripheral blood samples were obtained from patients with CAEBV. We investigated the host cellular gene expression profiles in CAEBV using a PCR array analysis that focused on T-cell and B-cell activation. We identified that four genes (IL-10, IL-2, IFNGR1, and INHBA) were upregulated in the CAEBV patients based on the PCR array analysis and the subsequent quantitative real-time PCR assay, where these four genes exhibited tenfold differences compared with the control subjects. These genes may be associated with inflammatory responses and with cell proliferation, and they may contribute to the development and progression of CAEBV.