Early differential diagnosis of malignant melanoma by abnormal DNA methylation

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In order to identify the early tumor marker of malignant melanoma, Sequenom MassARRAY quantitative methylation analysis using the MassARRAY Compact System was performed for the quantitative DNA methylation analysis of ZAR1 gene. Fifth surgical specimens (e.g. melanocytic nevus, Spitz nevus, dysplastic nevus, and melanoma in situ) that were similar to malignant melanoma by pathologic diagnosis were examined. DNA were collected by using Leica Laser Microdissection systems. However, the extraction of DNA and the quantitative DNA methylation analysis of ZAR1 gene using the MassARRAY were difficult, therefore we were not able to analyze.