The potential of pancreatic exocrine cells using for the donor source on islets transplantation

我们找到了适合基因转移的ngn3、Maf-A和PDX-1的基因顺序，并用荧光制作器制作了3种腺病毒载体。然后将每个腺病毒载体转染到AR42J细胞。经三次腺病毒转染后，长期培养均未发现存活细胞和转染基因的细胞。因此，我们制作了含有3个基因序列的腺病毒载体，将新载体转染到AR42J细胞上。少量存活细胞无荧光。我们推测新载体的大小影响了低的基因转移效率。同时，探讨了胰腺外分泌细胞的分离纯化方法。每隔一段时间，细胞存活数逐渐减少。同时也避免不了内分泌细胞尤其是β细胞的污染。

We found out the gene order each of the Ngn 3, Maf-A and PDX-1 suitable for the gene transfer, and made three adenovirus vectors with the fluorescence maker. Then each adenovirus Vectors were transfected to AR42J cells. After three adenovirus transfections, we could not find the survived and gene transfected cells on long-term culture.So we made the other adenovirus vector contained three gene orders, the new vector was transfected on AR42J cells. A few survived cells without fluorescence were found. We speculated that the size of the new vector affected the low gene transfer efficiency. Simultaneously, we tried to find out the method of the isolation and purification of exocrine cells of pancreas. After the each step,the number of survived cell decreased.Also we couldn't avoid from the Contamination of endocrine cells especially beta cells.