Investigation of the biological significance of collapsin response mediator protein (CRMP) in breast cancer

崩解蛋白反应介导蛋白（CRMP）在乳腺癌中的生物学意义的研究

• 批准号: 23591899
• 负责人: TAKASHI Ishikawa
• 金额: $ 1.58万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591899/
• 关键词: CRMP2; リン酸化型CRMP2; 乳癌; 乳腺; タキサン; 神経細胞軸索退縮因子; リン酸化CRMP2; CRMPs

The expression of CRMP2 mRNA was significantly decreased in breast cancer tissues, while that of the other CRMPs was similar between normal and breast cancer tissues. Immunohistochemistry revealed that CRMP2 protein expression was also decreased in breast cancer tissues (P < 0.001). Phosphorylated CRMP2 was observed in the nuclei of breast cancer cells but not in normal mammary cells (P < 0.001). Furthermore, nuclear phosphorylated CRMP2 expression was increased in proportion to the histological grade and triple-negative subtype. Reduced CRMP2 expression and elevated expression of nuclear phosphorylated CRMP2 may be associated with breast cancer progression.

CRMP2 mRNA在乳腺癌组织中的表达显著降低，而其他CRMPs mRNA在正常乳腺组织和乳腺癌组织中的表达无明显差异。免疫组化显示CRMP2蛋白在乳腺癌组织中的表达也明显降低（P < 0.001）。磷酸化的CRMP2在乳腺癌细胞核中表达，而在正常乳腺细胞核中未见表达（P < 0.001）。此外，核磷酸化CRMP2表达增加的比例，组织学分级和三阴性亚型。CRMP2表达降低和核磷酸化CRMP2表达升高可能与乳腺癌进展相关。

项目成果

Collapsin response mediator protein 2 is involved in regulating breast cancer progression

• DOI: 10.1007/s12282-013-0447-5
• 发表时间: 2014-11-01
• 期刊: BREAST CANCER
• 影响因子: 4
• 作者: Shimada, Kazuhiro;Ishikawa, Takashi;Goshima, Yoshio
• 通讯作者: Goshima, Yoshio