Analysis of amino acid-signal transduction in the intestinal system, applying a new nipple-bottle feeding system and artificial milk formula for newborn mice.

分析肠道系统中的氨基酸信号转导，为新生小鼠应用新型奶瓶喂养系统和人工奶粉。

• 批准号: 23591976
• 负责人: YOSHIDA KAZUHIRO
• 金额: $ 3.33万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591976/
• 关键词: アミノ酸; 腸管細胞増殖因子; 小腸大腸肛門外科学

We have presented that feeding neonatal mice with glutamine-deleted artificial milk formula induced high incidence of colonic hemorrhage due to the epithelial cell death, applying a new nipple-bottle feeding system for newborn mice. IEC6 rat intestinal epithelial cells cultured with low concentration of glutamine underwent apoptosis.Glutamine deprivation might cause instability of the intestinal epithelial alignment by apoptosis.

应用一种新的小鼠乳头瓶喂养系统，用谷氨酰胺缺失的人工奶粉喂养新生小鼠，导致新生小鼠因上皮细胞死亡而导致结肠出血的发生率较高。低浓度谷氨酰胺培养的IEC6大鼠肠上皮细胞发生凋亡，谷氨酰胺缺乏可能通过细胞凋亡导致肠上皮细胞排列不稳定。