The role of Necl-5 in the invasive activity of lung adenocarcinoma

在这项研究中，我们使用了细支气管肺泡癌（BAC）细胞系和成纤维细胞，以评估Necl-5的表达，在发展中的癌症间质通信，通过使用一个易于制备的双层胶原凝胶半球（DL-CGH）系统，使可视化的细胞迁移过程中的入侵。Necl-5在BAC细胞中的表达高于成纤维细胞。即使BAC细胞与成纤维细胞混合，这种趋势也没有改变。为了评估Necl-5在BAC细胞侵袭活性中的作用，我们使用RNA干扰（RNAi）敲低其表达。用DL-CGH进行的侵袭测定揭示了BAC细胞中Necl-5表达的抑制与被抑制的侵袭性相关。Necl-5在肺癌细胞中的表达对于它们在癌症-间质相互作用中的侵袭性至关重要，这表明Necl-5可能是抑制肺腺癌侵袭性的有利分子靶点。

In this study,we used a bronchioloalveolar carcinoma (BAC) cell line and fibroblasts to assess the expression of Necl-5 in the development of cancer–stroma communication by using an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) systemthat enables visualization of cellmigration during invasion. The expression of Necl-5 was higher in BAC cells than in fibroblasts. This tendency didn't change even when the BAC cells were mixed with fibroblasts. To assess the role of Necl-5 in the invasive activity of the BAC cells, we knocked down its expression using RNA interference (RNAi). The invasion assay with DL-CGH revealed that inhibition of Necl-5 expression in the BAC cells was associated with suppressed invasiveness. Necl-5 expression in lung cancer cells is crucial for their invasiveness in the cancer–stromal interaction, suggesting that Necl-5 could be a favorable molecular target for the suppression of invasiveness in lung adenocarcinoma.