Vasogenic brain edema in the spontaneous hypertensive rat

我们使用体外血脑屏障（BBB）模型，主要培养大鼠血脑屏障相关细胞。正常缺氧条件下，AGEs对血脑屏障模型的经内皮电阻（TEER）没有影响，但在3小时氧葡萄糖剥夺(OGD)/24小时再氧条件下，AGEs显著降低了TEER。西洛他唑抑制3 h OGD/24 h age复氧诱导的TEER下降。此外，虽然AGEs增加了细胞外转化生长因子(TGF)- β 1的产生，但西洛他唑抑制了细胞外TGF- β 1的产生，恢复了紧密连接的完整性。

We used in vitro blood-brain barrier (BBB) models with primarily cultured BBB-related cells from rats. Treatment of AGEs did not affect the transendothelial electrical resistance (TEER) in the BBB model under normoxia, but there was a significant decrease in TEER under 3-h oxygen glucose deprivation (OGD)/24-h reoxygenation conditions with AGEs. Cilostazol inhibited decreases in TEER induced by 3-h OGD/24-h reoxygenation with AGEs. Furthermore, while AGEs increased the production of extracellular transforming growth factor (TGF)-beta1, cilostazol inhibited the production of extracellular TGF- beta 1 and restored the integrity of tight junctions.