Mechanism of glioma invasion and its inhibition

胶质瘤侵袭及其抑制机制

• 批准号: 23592118
• 负责人: KITAI Ryuhei
• 金额: $ 2.83万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592118/
• 关键词: Glioma; Brain tumor; Invasion; Detection; グリオーマ; 浸潤先端; ５ＡＬＡ; サイトカイン; 脳腫瘍

The motility of cultured glioma cells were estimated by video monitor. The glioma cell line A172 was the most moving cells in other cells such as U87, T98G and primary glioma cell culture. The cells stimulated by low Oxygen chamber and low pH showed initial cell halt and following high motility. The inhibition of these motility was blocked by interferon beta. As for the detection of invasion front, recently 5ALA photodynamic diagnosis was introduced in glioma surgery. Using cultured cells, the fluorescent intensity and cell density were determined. The detection limit by the operator's naked eyes was reported.

通过视频监视器检测胶质瘤细胞的运动能力。胶质瘤细胞系A172在其他细胞如U87、T98G和原代胶质瘤细胞培养中运动最快。低氧室和低pH刺激的细胞表现出初始的细胞停滞和随后的高运动性。这些运动的抑制被干扰素β阻断。在浸润前沿的检测方面，近年来5ALA光动力学诊断被引入胶质瘤手术。使用培养的细胞，测定荧光强度和细胞密度。报告了操作员肉眼的检测限。