Catecholamine accelerates osteoblastic differentiation by enhancing bone -The effects of exercise on bone formation-

儿茶酚胺通过增强骨骼来加速成骨细胞分化 -运动对骨形成的影响 -

• 批准号: 23592227
• 负责人: SUZUKI Akinobu
• 金额: $ 3.16万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592227/
• 关键词: 骨形成蛋白; BMP; カテコラミン; 骨代謝; 骨形成蛋白(BMP); 骨形成; 骨形成蛋白（BMP）

Topical effects of a catecholamine on bone morphogenetic protein (BMP)-induced ectopic bone formation were investigated. Epinephrine enhanced bone induction by BMP-2. The mass of ossicles ectopically induced by BMP-2 was increased by the systemic addition of epinephrine. The expression level of alkaline phosphatase (ALP) in cells was consistently elevated by BMP-2 and was further elevated by the addition of epinephrine. The epinephrine-enhanced ALP elevation was specifically abolished by an antagonist to beta 2-adrenergic receptors and by a protein kinase A inhibitor. The luciferase activity of the Id1 gene was elevated by BMP-2 treatment and this activity was further enhanced by the addition of epinephrine. Epinephrine-enhanced luciferase activity was abolished by mutation of the cAMP-response element (CRE) sequence in the Id1 promoter, indicating that CRE-binding transcription proteins induced by epinephrine addition may act as enhancers of Smad-mediated BMP signaling.

研究了一种儿茶酚胺对骨形态发生蛋白（BMP）诱导的异位骨形成的局部作用。肾上腺素增强BMP-2的骨诱导作用。BMP-2异位诱导的听小骨质量通过全身添加肾上腺素而增加。碱性磷酸酶（ALP）在细胞中的表达水平持续升高，BMP-2和进一步升高，通过添加肾上腺素。肾上腺素增强的ALP升高被β 2-肾上腺素能受体拮抗剂和蛋白激酶A抑制剂特异性消除。通过BMP-2处理，Id 1基因的荧光素酶活性升高，并且通过加入肾上腺素进一步增强该活性。肾上腺素增强的荧光素酶活性被废除的突变cAMP反应元件（CRE）序列中的Id 1启动子，表明CRE-binding转录蛋白诱导肾上腺素除了可能作为Smad介导的BMP信号增强。

项目成果

日本ジュニアエリートテニス選手の世代別障害発生率の検討

日本青少年精英网球运动员代际残疾发生率调查

• 发表时间: 2013
• 作者: 橋本祐介;米谷泰一;奥平修三;平松久仁彦;中田研
• 通讯作者: 中田研

Prevalence and Motion Characteristics of Degenerative Cervical Spondylolisthesis in Symptomatic Adults

有症状成人退行性颈椎滑脱的患病率和运动特征

• 发表时间: 2013
• 作者: Suzuki A;Inoue H;Hayashi T;Nakamura H;Daubs MD;Wang JC
• 通讯作者: Wang JC

Clinical Outcomes of Microsurgical Bilateral Decompression via Unilateral Approach with Midline Spinous Process Splitting

中线棘突劈裂单侧入路显微手术双侧减压的临床结果

• 发表时间: 2013
• 作者: Suzuki A;Terai H;Toyoda H;Takahashi Y;Nakamura H
• 通讯作者: Nakamura H

BMP-2 Adverse Reactions Treated With Human Dose Equivalent Dexamethasone in a Rodent Model of Soft-Tissue Inflammation

• DOI: 10.1097/brs.0b013e31829cf348
• 发表时间: 2013-09
• 期刊: Spine
• 影响因子: 3
• 作者: C. Xiong;M. Daubs;S. Montgomery;B. Aghdasi;H. Inoue;Haijun Tian;A. Suzuki;Yanlin Tan;Tetsuo Hayashi;Monchai Ruangchainikom;Timothy Chai;M. Corey;Jeffrey C. Wang

正中神経剥離後に対する橈骨動脈穿通枝脂肪弁の使用経験

正中神经消融术后桡动脉穿支脂肪瓣的使用体会

• 发表时间: 2013
• 作者: 上村卓也;高松聖仁;岡田充弘;池田幹則;中村博亮
• 通讯作者: 中村博亮