Optical image analysis of anesthetic action on regional brain function and preparation of the video library of neuronal activities

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Optical imaging of neuronal functions, e.g., membrane excitation and intracellular calcium changes, in slices of the mouse cerebral cortex and hippocampus was performed using an imaging system equipped with a high-speed camera. The imaging applied to the characterization of neuronal functions under various conditions. Responses of the membrane excitation and intracellular calcium changes to stimulation were sensitively changed under various conditions of perfused solutions, particularly by temperature. Anesthetic actions of anesthetics on regional brain functions were referred to the location of the GABAA receptors. Different effect of anesthetics on the cortex and hippocampus may relate to the differences in localization of the GABAA receptor subtypes.The video images of neuronal functions obtained in this research project were collected and edited to produce a video library. The video images are up-loaded on the following website. URL: http://www.neuro-animation.jp/