Postoperative impairment of cognitive function in rats: A possible role of preoperative restraint stress induced microglial activation

大鼠认知功能的术后损伤：术前约束应激诱导小胶质细胞激活的可能作用

• 批准号: 23592273
• 负责人: YAMASHITA SOICHIRO
• 金额: $ 3.33万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592273/
• 关键词: 高次機能障害; 拘禁ストレス; 手術; spatial working memory; マイクログリア; IL-1β; Spatial working memory

This study was conducted to evaluate the effect of preoperative 2 hrs restraint stress on microglial activation, an expression of interleukin-1beta in hippocampus and spatial working memory after surgery in adult and aged rats. Spatial working memory was affected after surgery in a 6-8 months adult rat undergoing preoperative 2 hrs restraint stress. In a 18-20 months aged rat, surgery itself induced postoperative impairment of spatial working memory. However, the impairment continued even on the 14 postoperative days in an aged rat undergoing preoperative 2 hrs restraint stress. Those were associated with microglial activation and an increased expression of interleukin-1beta on neuron in hippocampus.

本研究旨在评价术前2小时约束应激对成年大鼠和老年大鼠术后小胶质细胞激活、海马白介素-1 β表达和空间工作记忆的影响。术前2小时约束应激对6 ~ 8月龄成年大鼠术后空间工作记忆的影响。在一只18-20个月大的大鼠中，手术本身引起了术后空间工作记忆的损伤。然而，在术前2小时约束应激的老年大鼠中，这种损伤甚至在术后14天仍在继续。这些与海马小胶质细胞激活和神经元上白细胞介素-1 β表达增加有关。