Study of the alteration with aging of human oocyte spindle function using extracted human oocyte in operation.

利用手术提取的人卵母细胞研究人卵母细胞纺锤体功能随衰老的变化。

• 批准号: 23592392
• 负责人: KUMAGAI Jin
• 金额: $ 3.24万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592392/
• 关键词: 減数紡錘体; 加齢; 中心体タンパク; ポロスコープ; 卵巣卵子採取

It was used extracted ovaries from eight patients of endometrial carcinoma stage I for this study. We obtained immature oocytes by the puncture on the follicles in ovaries. The number of obtained oocyte of a group of thirties (group A) was more than that of a group of forties (group B) significantly. After the culture in IVM medium, we obtained mature oocytes from immature oocytes of group A, but not from those of group B. It suggests the aging affects the oocyte maturation. We observed irregular arrangement of spindle but also regular one in immunofluorescence staining. The signal of microtubule localized near cell membrane intensely. It suggested the interaction between the microtubule and the cell membrane. The signal of pericentrin existed in contact with spindle and cell membrane and were not different between group A and B.From the above, we established the method that we obtained the available oocytes for investigations from extracted ovaries in operations.

本研究使用了八名 I 期子宫内膜癌患者的摘除卵巢。我们通过对卵巢中的卵泡进行穿刺来获得未成熟的卵母细胞。三十岁组（A组）获得的卵母细胞数量显着多于四十岁组（B组）。在IVM培养基中培养后，我们从A组的未成熟卵母细胞中获得了成熟的卵母细胞，但从B组的未成熟卵母细胞中获得了成熟的卵母细胞。这表明老化影响了卵母细胞的成熟。免疫荧光染色观察到纺锤体排列不规则，但也观察到规则排列。微管信号集中在细胞膜附近。它表明微管和细胞膜之间存在相互作用。中心周蛋白的信号存在于与纺锤体和细胞膜接触的位置，并且A组和B组之间没有差异。由此，我们建立了从手术中提取的卵巢中获取可用卵母细胞用于研究的方法。

项目成果

Suppression of hydatidiform molar growth by inhibiting endogenous brain–derived neurotrophic dactor / tyrosine kinase B signaling

通过抑制内源性脑源性神经营养因子/酪氨酸激酶 B 信号传导抑制葡萄胎生长

• 发表时间: 2012
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Shirasawa;H.;Kumagai;J.;Sato;W.;Kumazawa;Y.;Sato;N.;Terada;Y.;Kawamura K,Kumagai J et al
• 通讯作者: Kawamura K,Kumagai J et al

Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometorial carcinoma : a novel strategy for human oocyte research

从子宫内膜癌手术期间切除的卵巢中获取人类卵母细胞并进行体外成熟：人类卵母细胞研究的新策略

• 发表时间: 2013
• 期刊: J Assist Reprod Genet
• 作者: Shirasawa;H.;Kumagai;J.;Sato;W.;Kumazawa;Y.;Sato;N.;Terada;Y.
• 通讯作者: Y.

重症子宮内膜症不妊に対するUltra-long法の有効性の検討

超长法治疗重度子宫内膜异位症不孕症的有效性检查

• 发表时间: 2013
• 作者: Shirasawa;H.;Kumagai;J.;Sato;W.;Kumazawa;Y.;Sato;N.;Terada;Y.;Kawamura K,Kumagai J et al;熊谷仁,舘山奈江,白澤弘光,金森勝裕,金森恭子,熊澤由紀代,兒玉英也,寺田幸弘
• 通讯作者: 熊谷仁,舘山奈江,白澤弘光,金森勝裕,金森恭子,熊澤由紀代,兒玉英也,寺田幸弘

染色体異常を有する患者のART治療

染色体异常患者的ART治疗

• 发表时间: 2013
• 作者: Shirasawa;H.;Kumagai;J.;Sato;W.;Kumazawa;Y.;Sato;N.;Terada;Y.;Kawamura K,Kumagai J et al;熊谷仁,舘山奈江,白澤弘光,金森勝裕,金森恭子,熊澤由紀代,兒玉英也,寺田幸弘;熊谷仁
• 通讯作者: 熊谷仁