Ovarian cancer specific gene therapy by polymer-coated oncolytic adenovirus

由于溶瘤腺病毒，AdE3-midkine带负电荷，因此带正电荷的聚亚胺（PEI）和带负电荷的硫酸软骨素分别在AdE3-midkine表面层状分布。在抗腺病毒抗体中，12层PEI和硫酸软骨素的抗增殖作用最强。在B6C3F1同基因小鼠模型中，皮下和腹腔内肿瘤生长分别完全减少90%和60%。

Because oncolytic adenovirus, AdE3-midkine is negatively charged, positively charged polyethylenimine (PEI) and negatively charged chondroitin sulfate were respectively layered on the surface of AdE3-midkine. In the antiadenovirus antibody, 12 layers with PEI and chondroitin sulfate showed the most potent antiproliferative effect. In the B6C3F1 syngeneic mouse model, respectively 90% and 60% of subcutaneous and intraperitoneal tumor growth were completely reduced.