Mucosal immunity in the upper respiratory tract

上呼吸道粘膜免疫

• 批准号: 23592512
• 负责人: SUZUKI MASASHI
• 金额: $ 3.16万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592512/
• 关键词: 粘膜免疫; 樹状細胞; NKT細胞; 上気道; Th17細胞

The efficacy of alpha-galactosylceramide (aGalCer) as a mucosal adjuvant was examined. Mice were immunized intranasally with nontypeable Haemophilus influenzae (NTHi) P6 protein and a-GalCer. P6-specific antibody responses in the form of P6-specific IgA in nasal washes and serum IgG titers were significantly elevated. Splenic CD4+ T cells expressed P6-specific Th1 and Th2 cytokine mRNA. In addition, NTHi was quantified in nasal washes following NTHi challenges, and the clearance of NTHi from the nasopharynx was also enhanced. These results indicate that aGalCer might be an effective mucosal adjuvant.

检查了α-半乳糖神经酰胺（aGalCer）作为粘膜佐剂的功效。用不可分型的流感嗜血杆菌（NTHi）P6蛋白和α-GalCer鼻内免疫小鼠。鼻洗液中P6特异性伊加形式的P6特异性抗体应答和血清IgG滴度显著升高。脾脏CD 4 + T细胞表达P6特异性Th 1和Th 2细胞因子mRNA。此外，在NTHi激发后的鼻洗液中定量NTHi，并且NTHi从鼻咽的清除也增强。这些结果表明aGalCer可能是有效的粘膜佐剂。

项目成果

Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenza-specific long term mucosal immune responses in the nasopharynx

使用编码 P6 蛋白的质粒 DNA 和免疫刺激复合物进行鼻免疫可在鼻咽部引发不可分型的流感嗜血杆菌特异性长期粘膜免疫反应

• 发表时间: 2011
• 期刊: Vaccine
• 影响因子: 5.5
• 作者: Kodama S;Hirano T;Noda K;Umemoto S;Suzuki M
• 通讯作者: Suzuki M

Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and a-galactosylceramide

Th17 细胞有助于通过鼻部接种 P6 外膜蛋白和 a-半乳糖神经酰胺诱导的不可分型流感嗜血杆菌特异性保护性免疫

• 发表时间: 2011
• 期刊: Microbiol Immunol
• 影响因子: 2.6
• 作者: Noda K;Kodama S;Umemoto S;Nomi N;Hirano T;Suzuki M
• 通讯作者: Suzuki M

Monophosphoryl lipid A induced innate immune responses via TLR4 to enhance clearance of nontypeable Haemophilus influenzae and Moraxella catarrhalis fron the nasopharynx in mice

单磷酰脂质 A 通过 TLR4 诱导先天免疫反应，增强小鼠鼻咽部不可分型流感嗜血杆菌和卡他莫拉菌的清除

• 发表时间: 2011
• 期刊: FEMS immunol Med Microbiol
• 作者: Hirano T;Kodama S;Kawano T;Maeda K;Suzuki M
• 通讯作者: Suzuki M

上気道粘膜免疫におけるNKT細胞の機能解析

NKT细胞在上呼吸道黏膜免疫中的功能分析

• 作者: 梅本真吾;児玉悟;野田謙二;鈴木正志
• 通讯作者: 鈴木正志

Pili play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarrhalis

在卡他莫拉菌急性中耳炎小鼠模型中，菌毛在增强中耳细菌清除方面发挥着重要作用

• DOI: 10.1111/2049-632x.12025
• 发表时间: 2013
• 期刊: pathogens and disease
• 影响因子: 3.3
• 作者: Kawano T.,Hirano T.,Kodama S.;Mitsui MT.,Ahmed K.,Nishizono A.;Suzuki M
• 通讯作者: Suzuki M