Characterization of CFP-expressed Vav-deficient mice with spontaneous IOP elevation to evaluate pressure-dependent RGC death

尽管视神经病变本身也存在青光眼等严重眼病，但在体内研究视网膜神经节细胞（retinal ganglion cells，RGC）在相对高眼压下如何受到损伤并发展成神经病变的可视化系统似乎是至关重要的。因此，我们分别建立了表达青色荧光素蛋白（cyan fluorescein protein，CFP）的Vav 2缺陷型（CFP/Vav 2）小鼠和Vav 2，3缺陷型（CFP/Vav 2，3）小鼠。首先，我们研究并证明CFP/Vav 2小鼠系统允许我们在时间过程中定量和父性地评估RGCs在体内的损伤。这项创新很有希望，因为从RGC检查中获得的结果可用于大脑的神经细胞。

It seems critically useful to develop the visible system how the retinal ganglion cells (RGCs) suffer from its damages and proceed into neuropathy under relative high intraocular pressures in vivo, although serious ocular disease like glaucoma, exists itself in optic neuropathy. Therefore we generate cyan fluorescein protein (CFP) expressed Vav2-deficient (CFP/Vav2) mice and Vav2, 3-deficient (CFP/Vav2, 3) mice respectively that only RGCs express CFP in those mice. First, we examined and proved that CFP/Vav2 mice system allows us to evaluate the RGCs damages quantitatively and paternally in vivo on the time course. This innovation is promising because the results obtained from RGCs examination are available for the neural cells of the brain.