Regulation of ultraviolet-induced actiation of p38, epithelial-mesenchymal transition, and apoptosis in corneal epithelial cells

紫外线诱导的 p38 激活、上皮间质转化和角膜上皮细胞凋亡的调节

• 批准号: 23592591
• 负责人: KATO Naoko
• 金额: $ 3.33万
• 依托单位: National Defense Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592591/
• 关键词: 角膜上皮細胞; 紫外線; 上皮間葉系移行; アポトーシス; 酸化ストレス; 炎症

We irradiated ultraviolet onto the cultured corneal epithelial cells with various conditions. Phosphorylation of p38, decrease of E-cadherin, beta-catenin, and upregulation of a transcriptional factor, snail occurred, however, the representative mesenchymal markers were not induced.We suspected that ultraviolet alone was insufficient for induction. We also assessed the addition of cytokines (TGF-beta and/or TNF-alpha), however the combined stimuli with ultraviolet and cytokines did not success induction of epithelial mesenchymal transition characterized by upregulation of mesenchymal markers.

用紫外线照射不同条件下培养的角膜上皮细胞。p38的磷酸化，E-cadherin和β-catenin的减少，以及转录因子snail的上调发生，然而，代表性的间充质标志物没有被诱导。我们还评估了细胞因子（TGF-β和/或TNF-α）的添加，然而，紫外线和细胞因子的组合刺激没有成功诱导以间充质标志物上调为特征的上皮间充质转化。

项目成果

Intranuclear transition of b-catenin is requied for induction of epithelial-mesenchymal transition-like changes in cultured corneal epithelial cells.

β-连环蛋白的核内转变是诱导培养的角膜上皮细胞中上皮-间质转变样变化所必需的。

• 发表时间: 2012
• 作者: Kato N;Miyashita H;Okada N;Kawakita T;Yoshida S;Takeuchi M;Tsubota K;Shimmura S.
• 通讯作者: Shimmura S.

The role of p38 MAPK phosphorylated activation by reactive oxygen species in corneal epithelial cells

角膜上皮细胞中活性氧对 p38 MAPK 磷酸化激活的作用

• 发表时间: 2011
• 作者: Kato N;Kubota M;Miyashita H;Kawakita T;Yoshida S;Tsubota K;Shimmura S
• 通讯作者: Shimmura S

サイトカインと紫外線照射による角膜上皮細胞での完全型上皮間葉系移行の誘導

细胞因子和紫外线照射诱导角膜上皮细胞完全上皮间质转化

• 作者: 加藤直子;宮下英之;岡田直子;川北哲也;吉田悟;竹内大;坪田一男;榛村重人
• 通讯作者: 榛村重人