Research on the regeneration of tracheal cartilage using adipose-derived stem cell

本研究的目的是利用分化的脂肪干细胞（ADSC）制备的软骨细胞片构建圆柱形软骨。从白色兔的皮下脂肪组织中收获ADSC并培养以促进软骨细胞片的形成。虽然从ADSC的软骨细胞的分化最初是不令人满意的，软骨细胞片最终形成的几种生长因子的添加和培养条件的调整。然而，由于缺乏丰富的软骨基质，很难通过软骨细胞片的旋转孵育来制造圆柱形软骨。结论是，使用源自ADSC的软骨细胞制造无支架圆柱形软骨是极其困难的，并且适当的支架，例如细胞外基质，对于从干细胞再生气管软骨是必不可少的。

The aim of this study was to fabricate a cylindrical cartilage using cartilaginous cell sheets that were made by differentiated adipose-derived stem cells (ADSC). ADSCs were harvested from the subcutaneous adipose tissue of white rabbits and cultured in order to promote a formation of cartilaginous cell sheet. Although a differentiation from the ADSC to the cartilaginous cell was initially unsatisfactory, cartilaginous cell sheets were finally formed by the addition of several growth factors and the adjustment of culture conditions. However it was difficult to fabricate a cylindrical cartilage by a rotative incubation of cartilaginous cell sheets due to a lack of abundant cartilage matrix. It was concluded that a fabrication of a scaffold-free cylindrical cartilage using cartilaginous cells originated from the ADSCs was extremely difficult and adequate scaffold, such as extracellular matrix, was essential to a regeneration of tracheal cartilage from the stem cells.