Visualizing expression patterns of target genes in the gut of Adriamycin Mouse Model

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Background: Adriamycin mouse model (AMM) is a model of VACTERL anomalies. Various genes have been revealed affecting on those anomalies, but the precise reason of those is unclear. We aimed reveal the cause of those anomalies by staining some target genes, and realization of those genes presentation as 3D using new method. Methods: Time-mated CBA/Ca mice received two intraperitoneal injections of Adriamycin on embryonic day (E) 7 and 8. Fetuses were harvested from E9 to E11, stained following whole mount in situ hybridization with labeled RNA probes to detect Shh and Fork head box F1(Foxf1) transcripts. Immunolocalization with endoderm marker Hnf3b was used to visualize morphology. Embryos were scanned by Optical Projection Tomography that enables to obtain 3D representations of expressions. Conclusion: The delamination of the developing notochord is disrupted, which may influence signaling mechanisms from the notochord to the gut resulting in abnormal patterning of the gut.