Visualizing expression patterns of target genes in the gut of Adriamycin Mouse Model

阿霉素小鼠模型肠道中靶基因表达模式的可视化

• 批准号: 23592633
• 负责人: SATOU Hideaki
• 金额: $ 3.33万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592633/
• 关键词: 先天性消化器疾患学; VACTER Association; 食道閉鎖; 直腸肛門奇形; 脊索; 食道閉鎖症; アドリアマイシン; 期間食道瘻; アドリアマイシンマウス; 気管食道瘻

Background: Adriamycin mouse model (AMM) is a model of VACTERL anomalies. Various genes have been revealed affecting on those anomalies, but the precise reason of those is unclear. We aimed reveal the cause of those anomalies by staining some target genes, and realization of those genes presentation as 3D using new method. Methods: Time-mated CBA/Ca mice received two intraperitoneal injections of Adriamycin on embryonic day (E) 7 and 8. Fetuses were harvested from E9 to E11, stained following whole mount in situ hybridization with labeled RNA probes to detect Shh and Fork head box F1(Foxf1) transcripts. Immunolocalization with endoderm marker Hnf3b was used to visualize morphology. Embryos were scanned by Optical Projection Tomography that enables to obtain 3D representations of expressions. Conclusion: The delamination of the developing notochord is disrupted, which may influence signaling mechanisms from the notochord to the gut resulting in abnormal patterning of the gut.

背景：阿霉素小鼠模型（AMM）是一种VEGL异常模型。各种基因已被揭示影响这些异常，但这些的确切原因尚不清楚。我们的目的是通过对一些目标基因进行染色来揭示这些异常的原因，并利用新的方法实现这些基因的三维表达。 方法：CBA/Ca小鼠在胚胎第7天和第8天接受两次阿霉素腹腔注射。从E9至E11收获胎仔，在用标记的RNA探针进行整体原位杂交后染色，以检测Shh和Fork头盒F1（Foxf 1）转录物。使用内胚层标记物Hnf 3b的免疫定位来可视化形态。胚胎通过光学投影断层扫描进行扫描，从而能够获得表达的3D表示。结论：发育中的脊索的分层被破坏，这可能影响从脊索到肠道的信号传导机制，导致肠道的异常模式。

项目成果

Optical Projection Tomographyー遺伝子発現解析の新手法ー

光学投影断层扫描 - 基因表达分析的新方法 -

• 发表时间: 2012
• 作者: Uehara S;Oue T;Kawatsu M;Nara K;Fukuzawa M;佐藤英章
• 通讯作者: 佐藤英章

The long-term prognosis of two-flap anoplasty for mucosal prolapse following anorectoplasty for anal atresia.

肛门直肠成形术治疗肛门闭锁后粘膜脱垂二瓣成形术的长期预后。

• 发表时间: 2012
• 期刊: Pediatric Surgery International
• 影响因子: 1.8
• 作者: Sato H;Furuta S;Kawase H;Aoba T;Shima H;Wakisaka M;Kitagawa H.
• 通讯作者: Kitagawa H.

Effect of abnormal notochord delamination on hindgut development in the Adriamycin mouse model.

异常脊索分层对阿霉素小鼠模型后肠发育的影响。

• DOI: 10.1007/s00383-013-3386-5
• 发表时间: 2013
• 期刊: Pediatric Surgery International
• 影响因子: 1.8
• 作者: Hideaki Sato;Piotr Hajduk ;Shigeyuki Furuta;Munechika Wakisaka;Paula Murphy;Prem Puri;Hiroaki Kitagawa
• 通讯作者: Hiroaki Kitagawa

Optical Projection Tomography～遺伝子解析の新手法～

光学投影断层扫描〜遗传分析的新方法〜

• 发表时间: 2012
• 作者: Uehara S;Takama Y;Yoshida H;Hashii Y;Oue T;Usui N;佐藤英章
• 通讯作者: 佐藤英章

Tracheoinnominate artery fistula after laryngotracheal separation: prevention and management

• DOI: 10.1016/j.jpedsurg.2011.11.029
• 发表时间: 2012-02-01
• 期刊: JOURNAL OF PEDIATRIC SURGERY
• 影响因子: 2.4
• 作者: Sato, Hideaki;Kawase, Hirokazu;Kitagawa, Hiroaki
• 通讯作者: Kitagawa, Hiroaki