The role of protein phosphatase 2A in osteoblast differentiation and function

丝氨酸/苏氨酸蛋白磷酸酶2A(PP2A)调节细胞周期、细胞生长、细胞凋亡和信号转导等多种生理过程。PP2A由结构A亚基、调节B亚基和催化C亚基组成。在这项研究中，我们研究了PP2A Calpha在成骨细胞分化和功能中的表达和作用。在成骨细胞分化的初期，PP2A Calpha的表达和磷酸酶活性降低。为了阐明PP2A Calpha在成骨细胞分化中的作用，我们建立了PP2A Calpha敲除成骨细胞。沉默PP2A能显著增加成骨细胞的分化。我们的研究表明，PP2A Calpha通过骨相关基因的表达在骨形成和成骨细胞分化的调控中发挥重要作用。此外，PP2A Calpha在成骨细胞中的表达通过调控RANKL和OPG的表达来调节破骨细胞的形成。

Serine/threonine protein phosphatase 2A (PP2A) regulates several physiological processes such as the cell cycle, cell growth, apoptosis, and signal transduction. PP2A consists of a structural A subunit, a regulatory B subunit, and a catalytic C subunit. In this study, we examined the expression and role of PP2A Calpha in osteoblast differentiation and function. the expression and phosphatase activity of PP2A Calpha was decreased in the initial step of osteoblast differentiation. To clarify the role of PP2A Calpha in osteoblast differentiation, we established PP2A Calpha-knockdown osteoblasts. Silencing of PP2A Calphadramatically increased osteoblast differentiation. Our study demonstrated that PP2A Calpha plays important roles in the regulation of bone formation and osteoblast differentiation through the bone-related gene expressions. In addition, PP2A Calpha expression in osteoblasts modulates osteoclastogenesis by controlling the expressions of RANKL and OPG.