The relationship between local anesthetic effect of QX-314 and TRPV1 expression following inferior alveolar nerve transection in rats

QX-314局麻效果与大鼠下牙槽神经横断后TRPV1表达的关系

• 批准号: 23592730
• 负责人: KITAGAWA Junichi
• 金额: $ 3.33万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592730/
• 关键词: QX-314; TRPV1; カプサイシン; 神経障害性疼痛; 神経損傷; C 線維; 痛覚; 麻酔; 再生神経

QX-314 with capsaicin has been reported to produce long-lasting block of acute pain. However, little is reported of their effectiveness on neuropathic pain (NP) after nerve injury which involves various biophysical and molecular changes in sensory neurons. In this study the effect of this combination was evaluated under NP and non-NP conditions after inferior alveolar nerve (IAN) transection.The magnitude of QX-314 with capsaicin induced local anesthetic block varied under various conditions; however, no block was observed under NP conditions at 2 weeks after transection. Immunohistochemical study revealed that the number of regenerated neurons with transient receptor potential vanilloid 1 (TRPV1) expression gradually increased over time after transection. Unmyelinated neurons expressing TRPV1 regenerated slowly, especially in the presence of NP. In addition, TRPV1 expression shifted to myelinated fibers and this shift was more intense under NP than under non-NP conditions.

QX-314与辣椒素已被报道产生持久的急性疼痛阻滞。然而，很少有报道其对神经损伤后的神经病理性疼痛（NP）的有效性，这涉及感觉神经元的各种生物物理和分子变化。在这项研究中，在NP和非NP条件下，下牙槽神经（IAN）横断后，这种组合的效果进行了评估。QX-314与辣椒素诱导的局部麻醉阻滞的幅度在各种条件下变化;然而，在NP条件下，在横断后2周没有观察到阻滞。免疫组化结果显示，再生神经元瞬时受体电位香草酸1（TRPV 1）表达的数量随着时间的推移逐渐增加。表达TRPV 1的无髓神经元再生缓慢，尤其是在NP存在下。此外，TRPV 1的表达转移到有髓纤维，这种转移在NP条件下比在非NP条件下更强烈。

项目成果

のどごしについての生理学的考察．

喉咙啜饮的生理考虑。

• 发表时间: 2013
• 期刊: 日本味と匂学会誌
• 作者: Sasahira T;Kurihara M;Bhawal UK;Yamamoto K;Kirita T;Kuniyasu H.;北川純一，高辻華子，高橋功次郎，真貝富夫
• 通讯作者: 北川純一，高辻華子，高橋功次郎，真貝富夫

Facilitation of the swallowing reflex following administration of cannabinoids in rats.

给予大鼠大麻素后吞咽反射的促进。

• 发表时间: 2013
• 作者: Takatsuji H;Kitagawa J;Yamada Y;Yamamura K.
• 通讯作者: Yamamura K.

Upregulation of astroglia in the trigeminal motor nucleus involves in the modulation of the orofacial reflex after neruopathic pain

三叉神经运动核星形胶质细胞的上调参与神经病理性疼痛后口面部反射的调节

• 发表时间: 2012
• 作者: Mostafeezur RM;Kitagawa J;Yamada Y;Zakir HM;Yamamura K
• 通讯作者: Yamamura K

Evaluating the effect of QX-314 and TRPV1 expression following inferior alveolar nerve transection in rats.

评估大鼠下牙槽神经横断后 QX-314 和 TRPV1 表达的影响。

• 发表时间: 2012
• 作者: Zakir HM;Kitagawa J;Yamada Y;Mostafeezur RM;Yamamura K
• 通讯作者: Yamamura K

カンナビノイドは嚥下反射を促進する

大麻素促进吞咽反射

• 发表时间: 2011
• 作者: 北川純一;Mostafeezur RM;Zakir HM,山田好秋,山村健介
• 通讯作者: Zakir HM,山田好秋,山村健介