Analysis of molecular mechanism of muscle heterotopic ossification by forming a network with CCN family proteins

用肿瘤坏死因子-a处理小鼠成肌细胞系C2C12后，CCN2（又称结缔组织生长因子）的蛋白产量增加，肿瘤坏死因子-a是炎症时产生的。CCN2促进C2C12细胞增殖和MyoD蛋白的产生。与这些发现一致的是，体内ccn2缺失骨骼肌的分析显示PCNA染色降低和肌肉发育不全。此外，骨形态发生蛋白（BMP）2诱导的Runx2和Osterix基因表达水平在与CCN2共处理的C2C12细胞中显著降低。

The protein production of CCN2 (also known as Connective tissue growth factor) was increased in mouse myoblastic cell line C2C12 by treatment with tumor necrosis factor-a, which is produced upon inflammation. CCN2 promoted cell proliferation and the protein production of MyoD in C2C12 cells. Consistent with these findings, in vivo analyses with Ccn2-deficient skeletal muscle showed the decreased PCNA staining and muscle hypoplasia. Furthermore, bone morphogenetic protein (BMP)2-induced Runx2 and Osterix gene expression levels were significantly decreased in C2C12 cells co-treated with CCN2.