Isolation of thecandidate gene for the marker of the body time determinant in human saliva.

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We have identified 22 genes which are controlled directly by clock genes and show circadian transcription by the combination of ChIP on Chip and microarray experiments using human salivary gland cell line HSG. Rev-erb alpha was included in the 22 genes as a positive control. Then, 9 genes including Rev-erb alpha were selected as genes expressing in the normal human salivary gland by RT-PCR. In these 9 genes we found that 3 genes except Rev-erb alpha showed clear circadian transcription. Finally we have succeeded to identify the gene whose product is detected in saliva by western blot analysis. These results suggest that the selected gene is the good candidate for the marker of the body time determinant in human saliva.