Intercellular odontoblast-neuron communication to drive sensory transduction and mineralization sequences via ATP-permeable channel

细胞间成牙本质细胞-神经元通讯通过 ATP 渗透通道驱动感觉转导和矿化序列

• 批准号: 23592751
• 负责人: SHIBUKAWA Yoshiyuki
• 金额: $ 3.33万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592751/
• 关键词: 歯学; シグナル伝達; 神経科学; 象牙芽細胞; 細胞間コミュニケーション; 神経伝達; 痛覚; 歯内療法学; TRPチャネル群; 感覚受容; 歯痛

Mechanical-, thermal-, and chemical-stimuli to the surface of exposed dentin induce dentinal pain. However, mechanisms understanding dentinal pain remain to be clarified. We examined intercellular communication between odontoblasts and trigeminal ganglion (TG) neurons during direct mechanical stimulation of odontoblasts. The results showed that ATP released from mechanically stimulated odontoblasts via pannexin-1 in response to activation of mechano-sensitive transient receptor potential channels acts as neurotransmitter, to transduce a sensory signal to TG neurons by activation of ATP receptors. We suggest that odontoblasts are sensory receptor cells to drive sensory transduction sequence for dentinal pain.

露出牙本质表面的机械，热和化学刺激会引起牙本质疼痛。但是，理解牙本质疼痛的机制仍有待阐明。我们在直接机械刺激ODONTOPOLASTS的过程中检查了Odontoblasts和三叉神经节神经元之间的细胞间通信。结果表明，通过pannexin-1从机械刺激的牙胶细胞中释放出ATP，以响应机械敏感的瞬态受体电势通道的激活作为神经递质，从而通过激活ATP受体将感觉信号传递到TG神经元。我们建议牙胶细胞是驱动牙本质疼痛的感觉转导序列的感觉受体细胞。

项目成果

Magnetoencephalography Study of Right Parietal Lobe Dysfunction of the Evoked Mirror Neuron System in Antipsychotic-Free Schizophrenia

• DOI: 10.1371/journal.pone.0028087.s001
• 发表时间: 2011-01-01
• 期刊: Figshare
• 作者: Kato, Yutaka;Muramatsu, Taro;Mimura, Masaru
• 通讯作者: Mimura, Masaru

Voltage-dependent Sodium Channels and Calcium-activated Potassium Channels in Human Odontoblasts In Vitro

• DOI: 10.1016/j.joen.2012.06.015
• 发表时间: 2012-10-01
• 期刊: JOURNAL OF ENDODONTICS
• 影响因子: 4.2
• 作者: Ichikawa, Hideki;Kim, Hyong-Jung;Tazaki, Masakazu
• 通讯作者: Tazaki, Masakazu

Hyperosmotic Stress Induces Cell Death in an Odontoblast-lineage Cell Line

• DOI: 10.1016/j.joen.2012.03.023
• 发表时间: 2012-07-01
• 期刊: JOURNAL OF ENDODONTICS
• 影响因子: 4.2
• 作者: Fujisawa, Mari;Tokuda, Masayuki;Torii, Mitsuo
• 通讯作者: Torii, Mitsuo

Expression of TRPM8 and TRPA1 channels in rat odontoblasts, 11th International Confference on Tooth Morphogenesis and Differentiation

TRPM8和TRPA1通道在大鼠成牙本质细胞中的表达，第11届国际牙齿形态发生与分化会议

• 发表时间: 2013
• 作者: M Sato;M Tsumura;U Sobhan;M Tazaki,Y Shibukawa
• 通讯作者: M Tazaki,Y Shibukawa

歯科における感覚神経の臨床生理,In :歯科におけるしびれと痛みの臨床-歯科治療による神経損傷後の感覚神経障害,その対応とメカニズム−

牙科感觉神经的临床生理学，见：牙科临床麻木和疼痛 - 牙科治疗引起的神经损伤后的感觉神经病变，其反应和机制。

• 发表时间: 2011
• 作者: Andika C. Putra;Keiji Tanimoto;Elisna Syahruddin;Sita Andarini;Yoshio Hosoi;Keiko Hiyama;澁川義幸,市川秀樹(福田謙一,一戸達也,金子譲編)
• 通讯作者: 澁川義幸,市川秀樹(福田謙一,一戸達也,金子譲編)