Intercellular odontoblast-neuron communication to drive sensory transduction and mineralization sequences via ATP-permeable channel

暴露在牙本质表面的机械、热和化学刺激会引起牙本质疼痛。然而，理解牙本质疼痛的机制仍有待阐明。我们研究了成牙本质细胞和三叉神经节(TG)神经元在直接机械刺激成牙本质细胞时的细胞间通讯。结果表明，机械刺激的成牙本质细胞在机械敏感的瞬时受体电位通道被激活后，通过pAnnexin-1释放的ATP作为神经递质，通过激活ATP受体向三叉神经节神经元传递感觉信号。我们认为成牙本质细胞是驱动牙本质痛感觉转导序列的感觉受体细胞。

Mechanical-, thermal-, and chemical-stimuli to the surface of exposed dentin induce dentinal pain. However, mechanisms understanding dentinal pain remain to be clarified. We examined intercellular communication between odontoblasts and trigeminal ganglion (TG) neurons during direct mechanical stimulation of odontoblasts. The results showed that ATP released from mechanically stimulated odontoblasts via pannexin-1 in response to activation of mechano-sensitive transient receptor potential channels acts as neurotransmitter, to transduce a sensory signal to TG neurons by activation of ATP receptors. We suggest that odontoblasts are sensory receptor cells to drive sensory transduction sequence for dentinal pain.