The development of the cementum / periodontal membrane reproduction type implant with induced pluripotent stem cells derived from oral mucosa

口腔粘膜诱导多能干细胞牙骨质/牙周膜再生型种植体的开发

• 批准号: 23592905
• 负责人: TOYODA NAGATAKA
• 金额: $ 3.24万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592905/
• 关键词: 再生歯学; 口腔粘膜由来iPS細胞; 再生医療; セメント質・歯根膜再生型インプラント; iPS; ヒト口腔粘膜由来iPS細胞

Under existence of a lining-of-the-mouth origin iPS cell, Emdogain, and Matarigel, in co-culture with HAT-7 cell, The cell growth of an iPS cell stock, altered morphology, And existence of the Pax inducing factor in the rat odontogenic epithelial cell (HAT-7) surface and the inducible activity of Pax9 in the rat odontogenic epithelial cell (HAT-7) surface were accepted by the revelation and co-culture of Msx1, Pax9, and Lhx6 which are the transcription factors revealed in a dental generating process.Although the cell growth factor contained in Emdogain, Matigel, and GFR Matarigel, was comprehensively analyzed using the antibody array and the Bio-Plex suspension array system, it did not result in identification of a cell growth factor.

在与HAT-7细胞共培养的口腔粘膜来源的iPS细胞、Emdogain和Matarigel的存在下，iPS细胞储备物的细胞生长、改变的形态、实验结果表明，大鼠牙源性上皮细胞（HAT-7）表面存在Pax诱导因子，且Pax 9在HAT-7细胞表面具有诱导活性。虽然使用抗体阵列和Bio-Biological悬浮阵列系统全面分析了Emdogain、Matigel和GFR Matarigel中包含的细胞生长因子，但其未鉴定出细胞生长因子。