A new induction method for the controlled differentiation of human iPS cells using frozen sections

IPSC本身也有一些需要克服的问题。总之，为诱导分化和评价IPSCs的质量提供了一种简单有效的方法。在这项研究中，我们假设有可能通过在具有最适合诱导微环境的每个组织/器官的冰冻切片上培养来诱导IPSCs的受控分化。结果表明，肝上培养的IPSCs表达肝细胞相关标志的mRNA和蛋白，脑/脊髓培养的IPSCs分别表达神经元相关标志的mRNA和蛋白。此外，不同克隆的IPSCs在冰冻切片上的分化效率可能不同。因此，冰冻切片诱导人IPSCs是一种简单有效的方法，可用于IPSCs的诱导分化和评价IPSCs的潜能和/或质量。

iPSCs themselves have some problems that need to be overcome. Briefly, a simple and efficient method has been expected for the induction the differentiation and the evaluation of quality of iPSCs. In this study, we hypothesized that it is possible to induce the controlled differentiation of iPSCs by culturing on frozen sections of every tissue/organ which have the most suitable microenvironment for the induction. As a result, iPSCs which were cultured on liver expressed mRNA and protein for hepatocyte related markers, and iPSCs which were cultured on brain/spinal cord expressed mRNA and protein for neuron related markers, respectively. Moreover, the differentiation efficiency of iPSCs on frozen sections could be different among clones of iPSCs. Judging from these facts, the induction method of human iPSCs using frozen sections could be useful as a simple and effective measure for inducing the differentiation of iPSCs and evaluating the potential and/or quality of iPSCs.