Improvement of outcome of forebrain ischemia by use of dental pulp derived neurospheres

为了改善全脑缺血病理生理的预后，研究了牙髓组织侧群细胞形成的神经球对脑缺血病理生理的影响。脑细胞因子和脑源性神经元因子BDNF的表达也被检测为与神经球治疗相关的神经保护的预期机制。缺血后再灌注3小时后静脉植入神经球源性细胞可改善组织学结果。细胞因子和BDNF mRNA的表达在各组间无显著差异。据推测，神经球治疗的预后改善是由脑炎症过程或BDNF过表达以外的机制引起的。

To improve outcome of global cerebral ischemic pathophysiology, the effect of neurospheres, which was formed from side population cells in dental pulp tissue, were examined. Cerebral expressions of cytokines and BDNF, brain derived neuronal factor, were also examined as expected mechanisms of neuroprotection associated with neurosphere treatment. Post-ischemic intravenous implantation of neurosphere-derived cells after 3 hours of reperfusion improved histological outcome. However, mRNA expressions either of cytokines or BDNF were not significantly different among groups. It was surmised that outcome improvement from neurosphere treatment was caused by mechanisms other than cerebral inflammation process or BDNF overexpression.