Establishment of the newly bone regeneration therapy using polyhedra particles immobilized BMP-2 as a controlled release carrier for BMP-2

家蚕细胞病毒颗粒嵌入在微米大小的蛋白质晶体中，称为多面体，可以在恶劣的环境条件下保持感染性多年。但多角体的良好稳定性可用于组织工程细胞因子的缓释载体。在这里，我们展示了骨形态发生蛋白-2（BMP-2）包裹的多面体在临界大小的骨缺损中的完全愈合。尽管可吸收胶原海绵（ACS）安全有效地将重组人BMP-2（rhBMP-2）递送到愈合组织中，但目前的治疗方案以初始高速率释放rhBMP-2，之后速率迅速下降。用BMP-2多面体浸渍的ACS具有足够的成骨活性以促进临界尺寸的骨缺损的完全愈合，但是用高剂量的rhBMP-2浸渍的ACS显示不完全的骨愈合，这表明含有BMP-2的多面体微晶有望推进骨愈合的最新技术。

Bombyx mori cypovirus particles are embedded in micrometer-sized protein crystals called polyhedra and can remain infectious in harsh environmental conditions for years. But the remarkable stability of polyhedra can be applied on slow-release carriers of cytokines for tissue engineering. Here we show the complete healing in critical-sized bone defects by bone morphogenetic protein-2 (BMP-2) encapsulated polyhedra. Although absorbable collagen sponge (ACS) safely and effectively delivers recombinant human BMP-2 (rhBMP-2) into healing tissue, the current therapeutic regimens release rhBMP-2 at an initially high rate after which the rate declines rapidly. ACS impregnated with BMP-2 polyhedra had enough osteogenic activity to promote complete healing in critical-sized bone defects, but ACS with a high dose of rhBMP-2 showed incomplete bone healing, indicating that polyhedral microcrystals containing BMP-2 promise to advance the state of the art of bone healing.