Effects of early life stress on transcription or translation of developmental brain nerve cells

早期生活压力对发育脑神经细胞转录或翻译的影响

• 批准号: 23593031
• 负责人: MITSUHATA Chieko
• 金额: $ 3.33万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593031/
• 关键词: 発達; ストレス; 自閉症; 幼若期ストレス; 脳の発達

It is not clear about mechanism of pathogenic mechanism of mental disorder including autism. The purpose of this study is to elucidate the relation between early stress exposure and epigenetic gene regulations in the brain. It is also another purpose to explore the possibility that these epigenetic gene regulations exert influence to growth of brain development.There are some reports that early life events can induce long lasting changes in physiology and behavior.We chose Mother infant separation as early life stress.This separation starts from one day after birth for one week or two weeks.Corticosteron in the blood was measured. There was no significant difference between two groups.After this, we started another separation and total RNA samples of mesencephalon were prepared in seventh day after birth.New target genes were explored with DNA methylation and micro array. We did not get unique target genes.

包括孤独症在内的精神障碍的发病机制尚不清楚。本研究的目的是阐明早期应激暴露与大脑表观遗传基因调控的关系。本研究选择母婴分离作为早期生活应激的研究对象，从出生后第一天开始，持续一周或两周，测定血中皮质酮的含量。两组间差异无统计学意义，然后再次分离，于生后第7天取中脑组织制备总RNA，通过DNA甲基化和微阵列技术寻找新的靶基因。我们没有得到独特的靶基因。