Development of Safely Non-Invasive Dynamic Measurement Method of Extracellular Ions Using CCD-type Ion Image Sensor

我们利用增塑化聚氯乙烯膜（离子显微镜）开发了几种新型的ccd型离子图像传感器，用于监测细胞释放或被化学物质刺激吸附到细胞中的分析物离子的细胞外浓度。所研制的离子显微镜实际上可以应用于活细胞的显微观察。此外，我们还考察了增塑型聚氯乙烯膜在细胞培养过程中的抑制作用，得出了一些制备安全、无创膜的指导原则。

We developed several new CCD-type ion image sensors using plasticized poly(vinyl chloride) membrane (Ion Microscopes), in order to monitor extracellular concentration of analyte ions released from cells or adsorbed into cells by stimulation of chemicals. The ion microscope developed could be applied to micro observation of living cells actually. In addition, we examined inhibiting effect of plasticized poly(vinyl chloride) membranes during the cultivation of cells, and we obtained some guidelines of preparing safety and non-invasive membrane.