Analysis of chromatin function mediated by histone H4 lysine 20 methylation

组蛋白 H4 赖氨酸 20 甲基化介导的染色质功能分析

• 批准号: 23616009
• 负责人: ODA Hisanobu
• 金额: $ 3.41万
• 依托单位: National Hospital Organization, Kyushu Cancer Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23616009/
• 关键词: エピジェネティクス; 染色体; ヒストン修飾; ヒストンメチル化酵素; H4リジン20; 細胞周期; DNA複製; クロマチン; PR-Set7; ヒストン; メチル化; ヒストンメチル化; ヒストンメチルトランスフェラーゼ; set8; 遺伝子ノックアウトマウス

Histone post-translational modifications impact many aspects of chromatin and nuclear function. Histone H4 Lysine 20 methylation (H4K20me) has been implicated in regulating diverse processes ranging from the DNA damage response, chromatin condensation, and DNA replication to gene regulation. PR-Set7 is the sole enzyme that catalyzes monomethylation of H4K20. It is required for maintenance of all levels of H4K20me, and, importantly, loss of PR-Set7 is catastrophic for the various stages of development such as embryonic development and skin differentiation. These findings have placed PR-Set7, H4K20me, and proteins that recognize this modification as central nodes of many important pathways. The results were published in two journals, Genes & Development and EMBO Journal.

组蛋白翻译后修饰影响染色质和核功能的许多方面。组蛋白H4赖氨酸20甲基化（H4 K20 me）涉及调节从DNA损伤反应、染色质凝聚和DNA复制到基因调控的多种过程。PR-Set 7是唯一催化H4 K20单甲基化的酶。它是维持所有水平的H4 K20 me所必需的，重要的是，PR-Set 7的丢失对于发育的各个阶段如胚胎发育和皮肤分化是灾难性的。这些发现将PR-Set 7，H4 K20 me和识别这种修饰的蛋白质作为许多重要途径的中心节点。研究结果发表在《基因与发育》（Genes & Development）和《欧洲分子生物学组织杂志》（EMBO Journal）上。

项目成果

The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin.

• DOI: 10.1038/emboj.2011.421
• 发表时间: 2012-02-01
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Driskell, Iwona;Oda, Hisanobu;Blanco, Sandra;Nascimento, Elisabete;Humphreys, Peter;Frye, Michaela
• 通讯作者: Frye, Michaela

PR-Set7 and H4K20me1: at the crossroads of genomic integrity, cell cycle, chromosome condensation and transcription

PR-Set7 和 H4K20me1：处于基因组完整性、细胞周期、染色体浓缩和转录的十字路口

• 发表时间: 2012
• 期刊: Genes Dev
• 作者: Beck DB;Oda H;Shen SS;Reinberg DF
• 通讯作者: Reinberg DF

The role of PR-Set7 in replication licensing depends on Suv4-20h

• DOI: 10.1101/gad.195636.112
• 发表时间: 2012-12-01
• 期刊: GENES & DEVELOPMENT
• 影响因子: 10.5
• 作者: Beck, David B.;Burton, Adam;Reinberg, Danny
• 通讯作者: Reinberg, Danny